Anti-Myc tag抗体- ChIP Grade (ab9132)

Abreviews (6)Q&A (6)Specific References (66)

概述

  • 产品名称
    Anti-Myc tag抗体- ChIP Grade
    参阅全部 Myc tag 一抗
  • 描述
    山羊多克隆抗体to Myc tag - ChIP Grade
  • 宿主
    Goat
  • 特异性
    Antibody was verified by ELISA against peptide conjugated to BSA (EQKLISEEDL /BSA).
  • 经测试应用
    适用于: IP, ChIP, IHC-Fr, ICC/IF, WB, Flow Cyt, ICC, ELISAmore details
  • 免疫原

    Synthetic peptide:

    EQKLISEEDL

    (c-myc) conjugated to KLH.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.1% Sodium azide
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab9132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at 1-4 µg/mg of lysate.

Also PubMed: 20224777 

 
ChIP Use at an assay dependent concentration. ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads.
IHC-Fr 1/50.
ICC/IF Use at an assay dependent concentration.
WB 1/1000 - 1/30000. Can be blocked with Human c-Myc peptide (ab13837).
Flow Cyt Use at an assay dependent concentration. PubMed: 22158898

ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC 1/200 - 1/2000.
ELISA 1/100 - 1/500.

靶标

  • 相关性
    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • 细胞定位
    Nuclear
  • 别名
    • c-myc tag antibody
    • Myc Epitope Tag antibody

图片

  • Western blot - Anti-Myc tag antibody - ChIP Grade (ab9132)
    Western blot - Anti-Myc tag antibody - ChIP Grade (ab9132)
    All lanes : Anti-Myc tag antibody - ChIP Grade (ab9132) at 0.04 µg/ml

    Lane 1 : E. coli whole cell lysate at 0.2 µg
    Lane 2 : E. coli whole cell lysate at 0.1 µg
    Lane 3 : E. coli whole cell lysate at 0.05 µg
  • ChIP - Anti-Myc tag antibody - ChIP Grade (ab9132)
    ChIP - Anti-Myc tag antibody - ChIP Grade (ab9132)
    A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a Myc or His - tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).
  • Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody - ChIP Grade (ab9132)
    Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody - ChIP Grade (ab9132)This image is courtesy of an anonymous Abreview.
    ab9132, at 1/500, tagging the Myc epitope in human Hela cells by immunocytochemistry/ immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in 0.15% Triton prior to blocking in 10% serum for 1 hour at 23°C. The primary antibody incubated with the sample for 1 hour at 23°C. A donkey polyclonal anti-goat antibody, diluted 1/500, was used as the secondary.

    See Abreview

  • Immunohistochemistry (Frozen sections) - Anti-Myc tag antibody - ChIP Grade (ab9132)
    Immunohistochemistry (Frozen sections) - Anti-Myc tag antibody - ChIP Grade (ab9132)Image courtesy of anonymous abreviewer

    ab9132, at 1/50 tagging Myc epitope in zebrafish frozen skeleton section by immunohistochemistry. Tissue was Paraformaldehyde fixed and blocked with 5% BSA for 4 hours at 25oC. The sample was incubated with the primary antibody for 16 hours at 4oC followed by the secondary, Rabbit Anti-Goat Cy3® at 1/500 dilution.  

    See Abreview

文献

This product has been referenced in:
  • Fadeev A  et al. ALKALs are in vivo ligands for ALK family receptor tyrosine kinases in the neural crest and derived cells. Proc Natl Acad Sci U S A 115:E630-E638 (2018). Read more (PubMed: 29317532) »
  • Schou KB  et al. KIF13B establishes a CAV1-enriched microdomain at the ciliary transition zone to promote Sonic hedgehog signalling. Nat Commun 8:14177 (2017). Read more (PubMed: 28134340) »

See all 66 Publications for this product

发表研究结果有使用 ab9132？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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12 reviews or Q&A

10
Votes: Highest First

Thanks very much for your quick reply. I'm interested in the testing discount program you mentioned. Could you please let me know more about it?

You can find out more about the 100% Abreview Testing Discount Program below:

http://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount



For all the antibodies that you are intere...

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Dear Sir/Madam, I'm doing a ChIP experiment using drosophila tissue. Your ChIP-grade anti-epitope tag antibodies may be very helpful for me. The Abcam antibodies usually work well for us, so I would like to give it a try. But before we place a order, I...

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Thank you for contacting Abcam. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which gua...

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Will this antibody bind to endogenous c-myc protein? Do any anti-myc tag antibodies not bind to c-myc protein?

Thank you very much for your call today and for your question about ab9132 and our other myc tag antibodies.

I couldn't find any data specifically showing that ab9132 has been tested for reactivity against endogenous c-myc, however since the ...

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ChIP abreview for Anti-Myc tag antibody - ChIP Grade

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ChIP
Sample
Human Cell lysate - other (HEK cell Chromatin)
Specification
HEK cell Chromatin
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Detection step
Real-time PCR
Positive control
Histone H3 antibody
Negative control
IGG antibody, EV Control
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Abcam user community

Verified customer

提交于 Oct 29 2010

Western blot abreview for Anti-Myc tag antibody - ChIP Grade

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (293 cell)
Loading amount
20 µg
Specification
293 cell
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Aug 14 2009

Immunocytochemistry/ Immunofluorescence abreview for Anti-Myc tag antibody - ChIP Grade

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela)
Specification
Hela
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.15% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
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提交于 Aug 05 2009

Western blot abreview for Anti-Myc tag antibody - ChIP Grade

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (BY4741 strain)
Loading amount
100000 cells
Specification
BY4741 strain
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Oct 23 2007

Immunohistochemistry (Frozen sections) abreview for Anti-Myc tag antibody - ChIP Grade

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Zebrafish Tissue sections (skeleton)
Specification
skeleton
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Aug 17 2007

Will this antibody detect endogenous c-myc in mouse?

Thank you for your enquiry. Unfortunately the antibody has not been tested on endogenous mouse protein, so there is no data to say either way. I am sorry I could not be more helpful. Please do not hesitate to contact us if you need anything furt...

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ChIP abreview for Anti-Myc tag antibody - ChIP Grade

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ChIP
Sample
Human Cell lysate - whole cell (Jurkat cell lines)
Specification
Jurkat cell lines
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: formaldehyde
Detection step
Real-time PCR
Positive control
A stable Jurkat cell line expressing a myc- tagged version of rtTa-MYC.HBP1
Primers flanking the binding site of HBP1 on hCD2-LCR were used as a positive control
Negative control
As a negative control a Jurkat cell line that does not express the myc-tagged protein: rtTA was employed.
The HPRT gene was used as a negative control locus
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提交于 Nov 07 2006

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