Our Abpromise guarantee covers the use of ab9132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
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IP | Use at 1-4 µg/mg of lysate. Also PubMed: 20224777
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ChIP | Use at an assay dependent concentration. ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. | |
IHC-Fr | 1/50. | |
ICC/IF | Use at an assay dependent concentration. | |
WB | 1/1000 - 1/30000. Can be blocked with Human c-Myc peptide (ab13837). | |
Flow Cyt | Use at an assay dependent concentration. PubMed: 22158898 ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC | 1/200 - 1/2000. | |
ELISA | 1/100 - 1/500. |
ab9132, at 1/50 tagging Myc epitope in zebrafish frozen skeleton section by immunohistochemistry. Tissue was Paraformaldehyde fixed and blocked with 5% BSA for 4 hours at 25oC. The sample was incubated with the primary antibody for 16 hours at 4oC followed by the secondary, Rabbit Anti-Goat Cy3® at 1/500 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"