- 数据表
- 具体的参考文献 (33)
- 实验方案
概述
- 产品名称Anti-Myc tag抗体- ChIP Grade
参阅全部 Myc tag 一抗 - 描述山羊多克隆抗体to Myc tag - ChIP Grade
- 特异性Antibody was verified by ELISA against peptide conjugated to BSA (EQKLISEEDL /BSA).
- 经测试应用适用于: IP, ChIP, IHC-Fr, ICC/IF, WB, Flow Cyt, ICC, ELISAmore details
- 免疫原
Synthetic peptide:
EQKLISEEDL
(c-myc) conjugated to KLH.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
- 存储溶液Preservative: 0.1% Sodium azide
-
Concentration information loading... - 纯度Immunogen affinity purified
- 纯化说明Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- ChIP Related Products
- Compatible Secondaries
- Immunizing Peptide (Blocking)
应用
Our Abpromise guarantee covers the use of ab9132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | Use at 1-4 µg/mg of lysate. Also PubMed: 20224777
|
|
| ChIP | Use at an assay dependent concentration. ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. | |
| IHC-Fr | 1/50. | |
| ICC/IF | Use at an assay dependent concentration. | |
| WB | 1/1000 - 1/30000. Can be blocked with Human c-Myc peptide (ab13837). | |
| Flow Cyt | Use at an assay dependent concentration. PubMed: 22158898 ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| ICC | 1/200 - 1/2000. | |
| ELISA | 1/100 - 1/500. |
靶标
- 相关性Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
- 细胞定位Nuclear
-
别名
- avian myelocytomatosis viral oncogene homolog antibody
- bHLHe39 antibody
- c-Myc antibody
see all
Anti-Myc tag antibody - ChIP Grade 图像
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Western blot - Anti-Myc tag antibody - ChIP Grade (ab9132)All lanes : Anti-Myc tag antibody - ChIP Grade (ab9132) at 0.04 µg/ml
Lane 1 : E. coli whole cell lysate at 0.2 µg
Lane 2 : E. coli whole cell lysate at 0.1 µg
Lane 3 : E. coli whole cell lysate at 0.05 µg -
ChIP - Anti-Myc tag antibody - ChIP Grade (ab9132)A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a Myc or His - tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach). -
Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody - ChIP Grade (ab9132)This image is courtesy of an anonymous Abreview.ab9132, at 1/500, tagging the Myc epitope in human Hela cells by immunocytochemistry/ immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in 0.15% Triton prior to blocking in 10% serum for 1 hour at 23°C. The primary antibody incubated with the sample for 1 hour at 23°C. A donkey polyclonal anti-goat antibody, diluted 1/500, was used as the secondary. -
Immunohistochemistry (Frozen sections) - Anti-Myc tag antibody - ChIP Grade (ab9132)Image courtesy of anonymous abreviewerab9132, at 1/50 tagging Myc epitope in zebrafish frozen skeleton section by immunohistochemistry. Tissue was Paraformaldehyde fixed and blocked with 5% BSA for 4 hours at 25oC. The sample was incubated with the primary antibody for 16 hours at 4oC followed by the secondary, Rabbit Anti-Goat Cy3® at 1/500 dilution.
Anti-Myc tag antibody - ChIP Grade (ab9132)参考文献
This product has been referenced in:
- Asensio-Juan E et al. The histone demethylase PHF8 is a molecular safeguard of the IFN? response. Nucleic Acids Res 45:3800-3811 (2017). IP ; Human . Read more (PubMed: 28100697) »
- Street IH et al. Cytokinin acts through the auxin influx carrier AUX1 to regulate cell elongation in the root. Development 143:3982-3993 (2016). IP . Read more (PubMed: 27697901) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"