概述

  • 产品名称Anti-Myc tag抗体
    参阅全部 Myc tag 一抗
  • 描述
    兔多克隆抗体to Myc tag
  • 经测试应用适用于: WBmore details
  • 免疫原

    Synthetic peptide:

    C-EQKLISEEDL

    conjugated to KLH, corresponding to Myc tag.

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: E. coli.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab13836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 0.5 µg/ml. Can be blocked with Myc tag peptide.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
    • 细胞定位Nuclear
    • 别名
      • avian myelocytomatosis viral oncogene homolog antibody
      • bHLHe39 antibody
      • c-Myc antibody
      • class E basic helix-loop-helix protein 39 antibody
      • MRTL antibody
      • MYC antibody
      • Myc Epitope Tag antibody
      • myc proto-oncogene protein antibody
      • myc-related translation/localization regulatory factor antibody
      • oncogene c-Myc antibody
      • proto-oncogene c-Myc antibody
      • protooncogene homologous to myelocytomatosis virus antibody
      • transcription factor p64 antibody
      • v-myc avian myelocytomatosis viral oncogene homolog antibody
      • v-myc myelocytomatosis viral oncogene homolog (avian) antibody
      see all

    Anti-Myc tag antibody 图像

    • Anti-Myc tag antibody (ab13836) at 1 µg/ml + E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 1 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 41 kDa


      Exposure time : 10 seconds

      See Abreview

    Anti-Myc tag antibody (ab13836)参考文献

    This product has been referenced in:
    • Helbling M  et al. Investigation of IL-23 (p19, p40) and IL-23R identifies nuclear expression of IL-23 p19 as a favorable prognostic factor in colorectal cancer: a retrospective multicenter study of 675 patients. Oncotarget 5:4671-82 (2014). IHC . Read more (PubMed: 25015728) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab13836.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"