Anti-Myc tag抗体[9E10] (SureLight® Allophycocyanin) (ab72580)

概述

  • 产品名称
    Anti-Myc tag抗体[9E10] (SureLight® Allophycocyanin)
    参阅全部 Myc tag 一抗
  • 描述
    小鼠单克隆抗体[9E10] to Myc tag (SureLight® Allophycocyanin)
  • 偶联物
    SureLight® Allophycocyanin. Ex: 652nm, Em: 657nm
  • 特异性
    This antibody is specific for Myc tagged proteins. The Myc tag epitope (EQKLISEEDL) is located at the dimerization site of c-myc and therefore this antibody does not perform well at recognizing endogenous c-myc.
  • 经测试应用
    适用于: Flow Cyt, FRETmore details
  • 免疫原

    Synthetic peptide: AEEQKLISEE DLLRKRREQL KHKLE conjugated to KLH, corresponding to C terminal amino acids 408-432 of Human c-Myc.

  • 表位
    ab72580 recognizes the 9E10 epitope (EQKLISEEDL sequence) derived from the human c-myc protein.
  • 常规说明

    Excitation max. 652 nm. Emission max. 657.5 nm. F/P 1:1.

性能

应用

Our Abpromise guarantee covers the use of ab72580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration. ab73711-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FRET Use at an assay dependent concentration.

靶标

  • 相关性
    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • 细胞定位
    Nuclear
  • 别名
    • avian myelocytomatosis viral oncogene homolog antibody
    • bHLHe39 antibody
    • c-Myc antibody
    • class E basic helix-loop-helix protein 39 antibody
    • MRTL antibody
    • MYC antibody
    • Myc Epitope Tag antibody
    • myc proto-oncogene protein antibody
    • myc-related translation/localization regulatory factor antibody
    • oncogene c-Myc antibody
    • proto-oncogene c-Myc antibody
    • protooncogene homologous to myelocytomatosis virus antibody
    • transcription factor p64 antibody
    • v-myc avian myelocytomatosis viral oncogene homolog antibody
    • v-myc myelocytomatosis viral oncogene homolog (avian) antibody
    see all

文献

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