Anti-MYBBP1A抗体(ab93835)

概述

  • 产品名称Anti-MYBBP1A抗体
    参阅全部 MYBBP1A 一抗
  • 描述
    兔多克隆抗体to MYBBP1A
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 900 - 1000 of Human MYBBP1A.

  • 阳性对照
    • This antibody gave a positive signal in HeLa and HEK293 Whole Cell Lysates.

性能

应用

Our Abpromise guarantee covers the use of ab93835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 149 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能May activate or repress transcription via interactions with sequence specific DNA-binding proteins. Repression may be mediated at least in part by histone deacetylase activity (HDAC activity).
  • 细胞定位Cytoplasm. Nucleus. Nucleus > nucleolus. Shuttles between the nucleus and cytoplasm. Nuclear import may be mediated by KPNA2, while export appears to depend partially on XPO1/CRM1 (By similarity). Predominantly nucleolar.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AL024407 antibody
    • AU019902 antibody
    • cb486 antibody
    • FLJ37886 antibody
    • MBB1A_HUMAN antibody
    • MYB binding protein (P160) 1a antibody
    • myb binding protein (P160) 1a like antibody
    • Myb-binding protein 1A antibody
    • Mybbp1a antibody
    • nuclear protein P160 antibody
    • P160 antibody
    • p160MBP antibody
    • p53 activated protein 2 antibody
    • p67MBP antibody
    • PAP2 antibody
    • PAR interacting protein antibody
    • RP23 48A2.3 antibody
    • sb:cb486 antibody
    see all

Anti-MYBBP1A antibody 图像

  • All lanes : Anti-MYBBP1A antibody (ab93835) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 149 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes
  • ICC/IF image of ab93835 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93835, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 5µg/ml.

Anti-MYBBP1A antibody (ab93835)参考文献

ab93835 has not yet been referenced specifically in any publications.

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