概述

  • 产品名称Anti-MVP抗体[LMR5]
    参阅全部 MVP 一抗
  • 描述
    大鼠单克隆抗体[LMR5] to MVP
  • 经测试应用适用于: Flow Cyt, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Tissue/ cell preparation; 2R120 cell line, derived by stepwise Doxorubicin exposure of the SW-1573 non-small cell lung carcinoma cell line.

  • 阳性对照
    • Positive tissue - Cell Lines: Drug-sensitive parental cell lines and their multidrugresistant derivatives. Negative control antibody: Normal rat serum.

性能

应用

Our Abpromise guarantee covers the use of ab3374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
IHC-P
IHC-Fr
  • 应用说明Flow Cyt: use at a diluton of 1/20 - 1/30. Cell permeabilization required.
    IHC-P: use at a diluton of 1/20 - 1/30.
    IHC-Fr: use at a diluton of 1/20 - 1/30.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.
    • 组织特异性Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.
    • 序列相似性Contains 9 MVP (vault) repeats.
    • 结构域MVP 3 mediates interaction with PTEN.
      MVP 4 mediates interaction with PARP4.
    • 翻译后修饰Phosphorylated on Tyr residues after EGF stimulation.
      Dephosphorylated by PTPN11.
    • 细胞定位Cytoplasm. Nucleus > nuclear pore complex. 5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment.
    • Information by UniProt
    • 数据库链接
    • 别名
      • LRP antibody
      • Lung resistance related protein antibody
      • Lung resistance-related protein antibody
      • Major vault protein antibody
      • Major vault protein, rat, homolog of antibody
      • MVP antibody
      • MVP_HUMAN antibody
      • testicular secretory protein Li 30 antibody
      • VAULT 1 antibody
      • VAULT1 antibody
      see all

    Anti-MVP antibody [LMR5] (ab3374)参考文献

    This product has been referenced in:
    • Schroeijers AB  et al. Immunohistochemical detection of the human major vault protein LRP with two monoclonal antibodies in formalin-fixed, paraffin-embedded tissues. Am J Pathol 152:373-8 (1998). Read more (PubMed: 9466563) »

    See 1 Publication for this product

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    The Anti-MVP antibody [LMR5] (ab3374) is provided in the form of tissue culture supernatant. The exact concentration of the antibody present in the sample is therefore difficult to determine due to the other prot...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"