概述

  • 产品名称
  • 描述
    兔多克隆抗体to MVP
  • 经测试应用
    适用于: IHC-Fr, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Cat
  • 免疫原

    Recombinant fragment: EVV EIIQATIIRQ NQALRLRARK ECWDRDGKER VTGEEWLVTT VGAYLPAVFE EVLDLVDAVI LTEKTALHLR ARRNFRDFRG VSRRTGEEWL VTVQDTEAHV PDVHEEVLGV VPITTLGPHN YCVILDPVGP DGKNQLGQKR VVKGEKSFFL QPGEQLEQGI QDVYVLSEQQ GLLLRALQPL EEGEDEEKVS, corresponding to amino acids 158-350 of Human MVP (AAH15623)

  • 阳性对照
    • 293T, A431, H1299, HeLa, HepG2, MOLT4, A549 and Raji cells

性能

应用

Our Abpromise guarantee covers the use of ab97311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 22125622
WB 1/500 - 1/3000. Predicted molecular weight: 99 kDa.
IHC-P 1/100 - 1/500.

靶标

  • 功能
    Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.
  • 组织特异性
    Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.
  • 序列相似性
    Contains 9 MVP (vault) repeats.
  • 结构域
    MVP 3 mediates interaction with PTEN.
    MVP 4 mediates interaction with PARP4.
  • 翻译后修饰
    Phosphorylated on Tyr residues after EGF stimulation.
    Dephosphorylated by PTPN11.
  • 细胞定位
    Cytoplasm. Nucleus > nuclear pore complex. 5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment.
  • Information by UniProt
  • 数据库链接
  • 别名
    • LRP antibody
    • Lung resistance related protein antibody
    • Lung resistance-related protein antibody
    • Major vault protein antibody
    • Major vault protein, rat, homolog of antibody
    • MVP antibody
    • MVP_HUMAN antibody
    • testicular secretory protein Li 30 antibody
    • VAULT 1 antibody
    • VAULT1 antibody
    see all

图片

  • Anti-MVP antibody (ab97311) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size : 99 kDa
  • Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ab97311 at 1/500 dilution.

文献

This product has been referenced in:
  • Shi L  et al. Up-regulation of miR-146a increases the sensitivity of non-small cell lung cancer to DDP by downregulating cyclin J. BMC Cancer 17:138 (2017). WB . Read more (PubMed: 28202053) »
  • O'Dwyer D  et al. The proteomics of colorectal cancer: identification of a protein signature associated with prognosis. PLoS One 6:e27718 (2011). IHC-Fr ; Human . Read more (PubMed: 22125622) »

See all 2 Publications for this product

客户评价及客户问答

Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal Muscle)
Loading amount
30 µg
Specification
Skeletal Muscle
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

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Verified customer

提交于 Jun 30 2012

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