概述

  • 产品名称Anti-MVP抗体[1032]
    参阅全部 MVP 一抗
  • 描述
    小鼠单克隆抗体[1032] to MVP
  • 特异性This antibody reacts with a 104 to 110 kD protein known as Major Vault Protein (MVP).
  • 经测试应用适用于: WB, IP, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Proteins precipitated with AER317 from the phosphocellulose column flow through of extract of human breast cancer MCF-7 cells.

  • 阳性对照
    • Breast.
  • 常规说明


    MVP is identical to Lung-Resistance Related Protein (LRP). Treatment of cells with estradiol increases the amount of MVP in nuclear extract. Antibodies to progesterone and glucocorticoid receptors are also able to co-immunoprecipitate the MVP.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA
  • Concentration information loading...
  • 纯度IgG fraction
  • Primary antibody说明MVP is identical to Lung-Resistance Related Protein (LRP). Treatment of cells with estradiol increases the amount of MVP in nuclear extract. Antibodies to progesterone and glucocorticoid receptors are also able to co-immunoprecipitate the MVP.
  • 克隆单克隆
  • 克隆编号1032
  • 骨髓瘤unknown
  • 同种型IgG1
  • 轻链类型unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab2376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.
  • 组织特异性Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.
  • 序列相似性Contains 9 MVP (vault) repeats.
  • 结构域MVP 3 mediates interaction with PTEN.
    MVP 4 mediates interaction with PARP4.
  • 翻译后修饰Phosphorylated on Tyr residues after EGF stimulation.
    Dephosphorylated by PTPN11.
  • 细胞定位Cytoplasm. Nucleus > nuclear pore complex. 5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment.
  • Information by UniProt
  • 数据库链接
  • 别名
    • LRP antibody
    • Lung resistance related protein antibody
    • Lung resistance-related protein antibody
    • Major vault protein antibody
    • Major vault protein, rat, homolog of antibody
    • MVP antibody
    • MVP_HUMAN antibody
    • testicular secretory protein Li 30 antibody
    • VAULT 1 antibody
    • VAULT1 antibody
    see all

Anti-MVP antibody [1032] 图像

  • ab2376 - immunohistochemistry

    Formalin fixed paraffin embedded human breast carcinoma stained with MVP, using ABC and DAB chromogen.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue, staining MVP with ab2376.

Anti-MVP antibody [1032] (ab2376)参考文献

This product has been referenced in:
  • Xu H  et al. Knock-down of ubiquitin-specific protease 22 by micro-RNA interference inhibits colorectal cancer growth. Int J Colorectal Dis : (2011). WB ; Human . Read more (PubMed: 21773699) »
  • Silva P  et al. Tumor expression of major vault protein is an adverse prognostic factor for radiotherapy outcome in oropharyngeal carcinoma. Int J Radiat Oncol Biol Phys 69:133-40 (2007). IHC-P ; Human . Read more (PubMed: 17459603) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - other (Lung, endothelial cells)
Gel Running Conditions Reduced Denaturing (7.5 %)
Loading amount 40 µg
Specification Lung, endothelial cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Miss. Monica Giannotta

Verified customer

提交于 Jul 21 2015

I am very pleased to hear you would like to accept our offer and test ab2376 in Western blot on mouse. This code will give you: 1 free PRIMARY ANTIBODY OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview fo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"