概述

  • 产品名称
    Anti-MVP抗体[1032]
    参阅全部 MVP 一抗
  • 描述
    小鼠单克隆抗体[1032] to MVP
  • 特异性
    The clone number has been updated from (0.N.389) to (1032) both clone numbers name the same antibody clone.
  • 经测试应用
    适用于: Flow Cyt, IP, WB, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Proteins purified from extract of human breast cancer MCF-7 cells.

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 15mM Sodium Azide
    Constituents: 0.2% BSA, 10mM PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 克隆编号
    1032
  • 同种型
    IgG1
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab14562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use a concentration of 2 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 104-110 kDa.
IHC-P Use a concentration of 2 - 4 µg/ml.
EMSA Use at an assay dependent concentration.

靶标

  • 功能
    Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.
  • 组织特异性
    Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.
  • 序列相似性
    Contains 9 MVP (vault) repeats.
  • 结构域
    MVP 3 mediates interaction with PTEN.
    MVP 4 mediates interaction with PARP4.
  • 翻译后修饰
    Phosphorylated on Tyr residues after EGF stimulation.
    Dephosphorylated by PTPN11.
  • 细胞定位
    Cytoplasm. Nucleus > nuclear pore complex. 5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment.
  • Information by UniProt
  • 数据库链接
  • 别名
    • LRP antibody
    • Lung resistance related protein antibody
    • Lung resistance-related protein antibody
    • Major vault protein antibody
    • Major vault protein, rat, homolog of antibody
    • MVP antibody
    • MVP_HUMAN antibody
    • testicular secretory protein Li 30 antibody
    • VAULT 1 antibody
    • VAULT1 antibody
    see all

文献

This product has been referenced in:
  • Fowler PA  et al. In utero exposure to cigarette smoke dysregulates human fetal ovarian developmental signalling. Hum Reprod N/A:N/A (2014). Human . Read more (PubMed: 24847019) »
  • Bellingham M  et al. Exposure to chemical cocktails before or after conception - the effect of timing on ovarian development. Mol Cell Endocrinol 376:156-72 (2013). IHC . Read more (PubMed: 23791816) »

See all 4 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - other (monocytes)
Specification
monocytes
Blocking step
Roti-Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

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提交于 Jul 09 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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