Anti-MVP抗体[0.N.389] (ab14563)

概述

  • 产品名称Anti-MVP抗体[0.N.389]
    参阅全部 MVP 一抗
  • 描述
    小鼠单克隆抗体[0.N.389] to MVP
  • 经测试应用适用于: IHC-P, WB, Flow Cyt, IPmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Proteins purified from extract of human breast cancer MCF-7 cells.

  • 阳性对照
    • SKBR3 cells and breast carcinomas

性能

应用

Our Abpromise guarantee covers the use of ab14563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
WB
Flow Cyt
IP
EMSA
  • 应用说明Flow Cyt: Use at an assay dependent dilution.
    GSA: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 1 - 2 µg/ml for 30 min at RT (staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min).
    IP: Use 2µg/mg protein lysate (Use Protein G).
    WB: Use at a concentration of 1 - 2 µg/ml for 2 hrs at RT. Predicted molecular weight: 110 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.
    • 组织特异性Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.
    • 序列相似性Contains 9 MVP (vault) repeats.
    • 结构域MVP 3 mediates interaction with PTEN.
      MVP 4 mediates interaction with PARP4.
    • 翻译后修饰Phosphorylated on Tyr residues after EGF stimulation.
      Dephosphorylated by PTPN11.
    • 细胞定位Cytoplasm. Nucleus > nuclear pore complex. 5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment.
    • Information by UniProt
    • 数据库链接
    • 别名
      • LRP antibody
      • Lung resistance related protein antibody
      • Lung resistance-related protein antibody
      • Major vault protein antibody
      • Major vault protein, rat, homolog of antibody
      • MVP antibody
      • MVP_HUMAN antibody
      • testicular secretory protein Li 30 antibody
      • VAULT 1 antibody
      • VAULT1 antibody
      see all

    Anti-MVP antibody [0.N.389] (ab14563)参考文献

    ab14563 has not yet been referenced specifically in any publications.

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