Anti-MUSK抗体(ab92950)

概述

  • 产品名称Anti-MUSK抗体
    参阅全部 MUSK 一抗
  • 描述
    兔多克隆抗体to MUSK
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    不与反应: Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human MUSK.

  • 阳性对照
    • This antibody gave a positive signal in Human and Mouse Skeletal Muscle Tissue Lysates.

性能

应用

Our Abpromise guarantee covers the use of ab92950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 97 kDa).

靶标

  • 功能Receptor tyrosine kinase that is a key mediator of agrin's action and is involved in neuromuscular junction (NMJ) organization.
  • 疾病相关Defects in MUSK is a cause of congenital myasthenic syndrome with acetylcholine receptor deficiency (CMS-ACHRD) [MIM:608931]. A post-synaptic congenital myasthenic syndrome. Mutations underlying AChR deficiency cause a 'loss of function' and show recessive inheritance. Note=MUSK mutations lead to decreased agrin-dependent AChR aggregation, a critical step in the formation of the neuromuscular junction.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family.
    Contains 1 FZ (frizzled) domain.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 翻译后修饰Ubiquitinated by PDZRN3. Ubiquitination promotes endocytosis and lysosomal degradation.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MDK 4 antibody
    • MDK4 antibody
    • MGC126323 antibody
    • MGC126324 antibody
    • Muscle antibody
    • Muscle skeletal receptor tyrosine kinase antibody
    • Muscle skeletal receptor tyrosine protein kinase antibody
    • Muscle specific kinase receptor antibody
    • Muscle specific tyrosine kinase receptor antibody
    • Muscle specific tyrosine protein kinase receptor antibody
    • Muscle-specific kinase receptor antibody
    • Muscle-specific tyrosine-protein kinase receptor antibody
    • MuSK antibody
    • MUSK_HUMAN antibody
    • Neural fold somite kinase 1 antibody
    • Neural fold somite kinase 2 antibody
    • Neural fold somite kinase 3 antibody
    • Neural fold somite kinase1 antibody
    • Neural fold somite kinase2 antibody
    • Neural fold somite kinase3 antibody
    • Nsk 1 antibody
    • Nsk 2 antibody
    • Nsk 3 antibody
    • Nsk1 antibody
    • Nsk2 antibody
    • Nsk3 antibody
    • Receptor tyrosine kinase MuSK antibody
    • Skeletal muscle receptor tyrosine kinase antibody
    • skeletal receptor tyrosine-protein kinase antibody
    see all

Anti-MUSK antibody 图像

  • All lanes : Anti-MUSK antibody (ab92950) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 97 kDa
    Observed band size : 105 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

Anti-MUSK antibody (ab92950)参考文献

ab92950 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (HEK293 cells transfected with rat MuSK)
Loading amount 30 µg
Specification HEK293 cells transfected with rat MuSK
Gel Running Conditions Reduced Denaturing (7,5% polyacrylamide)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Sep 06 2012

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Je suis ravi que vous souhaitiez participer à notre offre et tester ab92950 chez le rat (80% d'homologie entre l'immunogène et la protéine de rat, voir fic...

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