Anti-Muscarinic Acetylcholine Receptor M4抗体- Carboxyterminal end (ab41170)

概述

  • 产品名称Anti-Muscarinic Acetylcholine Receptor M4抗体- Carboxyterminal end
    参阅全部 Muscarinic Acetylcholine Receptor M4 一抗
  • 描述
    兔多克隆抗体to Muscarinic Acetylcholine Receptor M4 - Carboxyterminal end
  • 特异性Cross reactivity for ab41170: M4 Muscarinic Receptor (469-478): 100% M4 Muscarinic Receptor: 70% No cross reactivity was observed in: M1 Muscarinic Receptor(451-460) M1 Muscarinic Receptor M2 Muscarinic Receptor(457-466) M2 Muscarinic Receptor M3 Muscarinic Receptor(580-589) M3 Muscarinic Receptor M5 Muscarinic Receptor(519-531) M5 Muscarinic Receptor
  • 经测试应用适用于: ICC, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    peptide analogue of the C terminal of rat muscarinic M4 receptor covalently attached onto a carrier protein.

  • 阳性对照
    • Rat brain tissue, whole brain homogenate and cultured cells.

性能

应用

Our Abpromise guarantee covers the use of ab41170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC
WB
ELISA
  • 应用说明ELISA: Use at an assay dependent dilution.
    ICC: 1/500.
    WB: 1/400. Predicted molecular weight: 53 kDa.
    This antibody is very sensitive to blocking agent. We recommend using whole goats serum for optimal results. Avoid any bovine products (BSA and milk).

    With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and postassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, mouse studies link its function to adenylyl cyclase inhibition. Muscarinic acetylcholine receptor M4 has been reported to be expressed in many regions of the rat brain, particularly the striatum and hippocampus.
    • 细胞定位Cell Membrane
    • 数据库链接
    • 别名
      • AChR antibody
      • Cholinergic receptor muscarinic 4 antibody
      • Chrm 4 antibody
      • CHRM4 antibody
      • HM 4 antibody
      • HM4 antibody
      • M4 antibody
      • Muscarinic acetylcholine receptor M4 antibody
      see all

    Anti-Muscarinic Acetylcholine Receptor M4 antibody - Carboxyterminal end (ab41170)参考文献

    This product has been referenced in:
    • Falsafi SK  et al. Scopolamine administration modulates muscarinic, nicotinic and NMDA receptor systems. PLoS One 7:e32082 (2012). WB ; Mouse . Read more (PubMed: 22384146) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab41170.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"