Anti-Muscarinic Acetylcholine Receptor M4抗体[18C7.2] (ab77956)

概述

  • 产品名称Anti-Muscarinic Acetylcholine Receptor M4抗体[18C7.2]
    参阅全部 Muscarinic Acetylcholine Receptor M4 一抗
  • 描述
    小鼠单克隆抗体[18C7.2] to Muscarinic Acetylcholine Receptor M4
  • 特异性ab77956 reacts with Muscarinic acetylcholine receptor M4. There is no reactivity with the other subtypes.
  • 经测试应用适用于: IHC-Fr, Flow Cyt, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Monkey
  • 免疫原

    Muscarinic Acetylcholine Receptor M4 i3 loop (human), fused to a tag.

  • 阳性对照
    • Transfected CHO cell lines

性能

应用

Our Abpromise guarantee covers the use of ab77956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent dilution.
Flow Cyt Use 1µg for 106 cells.
WB Use at an assay dependent dilution. Predicted molecular weight: 53 kDa.
IP Use at an assay dependent dilution.

靶标

  • 相关性The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and postassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, mouse studies link its function to adenylyl cyclase inhibition. Muscarinic acetylcholine receptor M4 has been reported to be expressed in many regions of the rat brain, particularly the striatum and hippocampus.
  • 细胞定位Cell Membrane
  • 数据库链接
  • 别名
    • AChR antibody
    • Cholinergic receptor muscarinic 4 antibody
    • Chrm 4 antibody
    • CHRM4 antibody
    • HM 4 antibody
    • HM4 antibody
    • M4 antibody
    • Muscarinic acetylcholine receptor M4 antibody
    see all

Anti-Muscarinic Acetylcholine Receptor M4 antibody [18C7.2] 图像

  • Overlay histogram showing SH-SY5Y cells stained with ab77956 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77956, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Muscarinic Acetylcholine Receptor M4 antibody [18C7.2] (ab77956)参考文献

This product has been referenced in:
  • Mei F  et al. Accelerated remyelination during inflammatory demyelination prevents axonal loss and improves functional recovery. Elife 5:N/A (2016). IHC-FoFr ; Mouse . Read more (PubMed: 27671734) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293, HGT101)
Loading amount 10 µg
Specification HEK293, HGT101
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Rob Tolhurst

Verified customer

提交于 Sep 16 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (STC1)
Loading amount 10 µg
Specification STC1
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Rob Tolhurst

Verified customer

提交于 Sep 16 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"