Anti-Muscarinic Acetylcholine Receptor 2抗体[B8E5] (ab90805)

概述

  • 产品名称Anti-Muscarinic Acetylcholine Receptor 2抗体[B8E5]
    参阅全部 Muscarinic Acetylcholine Receptor 2 一抗
  • 描述
    小鼠单克隆抗体[B8E5] to Muscarinic Acetylcholine Receptor 2
  • 经测试应用适用于: WB, IHC-Fr, Functional Studies, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Guinea pig, Human
    预测可用于: Cow, Pigeon, Pig, Chimpanzee, Pufferfish (Fugu), Zebrafish, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to N-terminal part of the second extracellular loop, amino acids 168-192 (

    VRTVEDGECYIQFFSNAAVTFGTAI

    ) of Human Muscarinic Acetylcholine Receptor 2.

  • 表位ab90805 recognizes the pentapeptide VRTVE (amino acids 168-172)
  • 常规说明


    ab90805 inhibits the ß-adrenergic L-type Ca2+ currents through activation of the muscarinic acetylcholine receptor M2. It suggests that the antibody increases cGMP. ab90805 acts by functional dimerization of the receptor resulting in stabilization of the constitutive active receptor dimers and induces a small decrease in carbachol affinity for the M2 receptor.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • 纯化说明0.2 µm filtered
  • Primary antibody说明ab90805 inhibits the ß-adrenergic L-type Ca2+ currents through activation of the muscarinic acetylcholine receptor M2. It suggests that the antibody increases cGMP. ab90805 acts by functional dimerization of the receptor resulting in stabilization of the constitutive active receptor dimers and induces a small decrease in carbachol affinity for the M2 receptor.
  • 克隆单克隆
  • 克隆编号B8E5
  • 同种型IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab90805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50. Predicted molecular weight: 52 kDa.
IHC-Fr 1/50.
Functional Studies Use at an assay dependent concentration.
ICC/IF 1/50.

靶标

  • 功能The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.
  • 疾病相关Genetic variations in CHRM2 can influence susceptibility to major depressive disorder (MDD) [MIM:608516]. MDD is one of the most common psychiatric disorders. MDD is a complex trait characterized by one or more major depressive episodes without a history of manic, mixed, or hypomanic episodes. A major depressive episode is characterized by at least 2 weeks during which there is a new onset or clear worsening of either depressed mood or loss of interest or pleasure in nearly all activities. Four additional symptoms must also be present including changes in appetite, weight, sleep, and psychomotor activity; decreased energy; feelings of worthlessness or guilt; difficulty thinking, concentrating, or making decisions; or recurrent thoughts of death or suicidal ideation, plans, or attempts. The episode must be accompanied by distress or impairment in social, occupational, or other important areas of functioning.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.
  • 细胞定位Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 7TM receptor antibody
    • Acetylcholine receptor, muscarinic, 2 antibody
    • AChR antibody
    • Acm2 antibody
    • ACM2_HUMAN antibody
    • Cholinergic receptor muscarinic 2 antibody
    • Cholinergic receptor, muscarinic 2, cardiac antibody
    • Cholinergic receptor, muscarinic 2, isoform a antibody
    • Cholinergic receptor, muscarinic 2a antibody
    • CHRM 2 antibody
    • CHRM2 antibody
    • chrm2a antibody
    • CM2 antibody
    • FLJ43243 antibody
    • HM 2 antibody
    • HM2 antibody
    • M2 antibody
    • M2 muscarinic receptor antibody
    • M2-mAChR antibody
    • MGC120006 antibody
    • MGC120007 antibody
    • Muscarinic acetylcholine receptor M2 antibody
    • Muscarinic M2 receptor antibody
    see all

Anti-Muscarinic Acetylcholine Receptor 2 antibody [B8E5] (ab90805)参考文献

This product has been referenced in:
  • Mei F  et al. Accelerated remyelination during inflammatory demyelination prevents axonal loss and improves functional recovery. Elife 5:N/A (2016). IHC-FoFr ; Mouse . Read more (PubMed: 27671734) »
  • Nascimento JH  et al. cGMP-mediated inhibition of cardiac L-type Ca(2+) current by a monoclonal antibody against the M(2) ACh receptor. Am J Physiol Cell Physiol 281:C1251-8 (2001). Read more (PubMed: 11546662) »

See all 3 Publications for this product

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Unfortunately we do not have blocking peptides for any of the antibody ab2805, ab90805 and ab109226. I apologize for the inconvenience this may have caused.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"