Anti-Muscarinic Acetylcholine Receptor 2抗体(ab109050)

概述

  • 产品名称Anti-Muscarinic Acetylcholine Receptor 2抗体
    参阅全部 Muscarinic Acetylcholine Receptor 2 一抗
  • 描述
    兔多克隆抗体to Muscarinic Acetylcholine Receptor 2
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Sheep, Rabbit, Cow, Cat, Pig, Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human Muscarinic Acetylcholine Receptor 2.

  • 阳性对照
    • This antibody gave a positive signal in Human Brain tissue lysate as well as SHSY5Y whole cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab109050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa). Abcam recommends using milk as the blocking agent (3%).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.
  • 疾病相关Genetic variations in CHRM2 can influence susceptibility to major depressive disorder (MDD) [MIM:608516]. MDD is one of the most common psychiatric disorders. MDD is a complex trait characterized by one or more major depressive episodes without a history of manic, mixed, or hypomanic episodes. A major depressive episode is characterized by at least 2 weeks during which there is a new onset or clear worsening of either depressed mood or loss of interest or pleasure in nearly all activities. Four additional symptoms must also be present including changes in appetite, weight, sleep, and psychomotor activity; decreased energy; feelings of worthlessness or guilt; difficulty thinking, concentrating, or making decisions; or recurrent thoughts of death or suicidal ideation, plans, or attempts. The episode must be accompanied by distress or impairment in social, occupational, or other important areas of functioning.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.
  • 细胞定位Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 7TM receptor antibody
    • Acetylcholine receptor, muscarinic, 2 antibody
    • AChR antibody
    • Acm2 antibody
    • ACM2_HUMAN antibody
    • Cholinergic receptor muscarinic 2 antibody
    • Cholinergic receptor, muscarinic 2, cardiac antibody
    • Cholinergic receptor, muscarinic 2, isoform a antibody
    • Cholinergic receptor, muscarinic 2a antibody
    • CHRM 2 antibody
    • CHRM2 antibody
    • chrm2a antibody
    • CM2 antibody
    • FLJ43243 antibody
    • HM 2 antibody
    • HM2 antibody
    • M2 antibody
    • M2 muscarinic receptor antibody
    • M2-mAChR antibody
    • MGC120006 antibody
    • MGC120007 antibody
    • Muscarinic acetylcholine receptor M2 antibody
    • Muscarinic M2 receptor antibody
    see all

Anti-Muscarinic Acetylcholine Receptor 2 antibody 图像

  • All lanes : Anti-Muscarinic Acetylcholine Receptor 2 antibody (ab109050) at 1 µg/ml (Milk blocking - 3%)

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 51 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 67 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

Anti-Muscarinic Acetylcholine Receptor 2 antibody (ab109050)参考文献

ab109050 has not yet been referenced specifically in any publications.

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