Anti-muscarinic Acetylcholine Receptor 1抗体(ab75178)

概述

  • 产品名称Anti-muscarinic Acetylcholine Receptor 1抗体
    参阅全部 Muscarinic Acetylcholine Receptor 1 一抗
  • 描述
    兔多克隆抗体to muscarinic Acetylcholine Receptor 1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide derived from the internal region of human Muscarinic Acetylcholine Receptor 1

  • 阳性对照
    • Extracts from HepG2 cells

性能

应用

Our Abpromise guarantee covers the use of ab75178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 51 kDa.
ELISA 1/5000.

靶标

  • 相关性The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. Muscarinic receptors are so called as they are more sensitive to the effects of the alkaloid muscarine, rather than nicotine.
  • 细胞定位Cell Membrane; multi-pass membrane protein
  • 数据库链接
  • 别名
    • AChR antibody
    • Cholinergic receptor muscarinic 1 antibody
    • CHRM1 antibody
    • HM1 antibody
    • M1 antibody
    • Muscarinic acetylcholine receptor M1 antibody
    see all

Anti-muscarinic Acetylcholine Receptor 1 antibody 图像

  • All lanes : Anti-muscarinic Acetylcholine Receptor 1 antibody (ab75178) at 1/500 dilution

    Lane 1 : extracts from HepG2 cells
    Lane 2 : extracts from HepG2 cells with immunizing peptide at 5 µg

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 51 kDa
    Observed band size : 51 kDa

    Secondary antibody - anti-rabbit HRP (ab6721)

Anti-muscarinic Acetylcholine Receptor 1 antibody (ab75178)参考文献

This product has been referenced in:
  • Falsafi SK  et al. Scopolamine administration modulates muscarinic, nicotinic and NMDA receptor systems. PLoS One 7:e32082 (2012). WB ; Mouse . Read more (PubMed: 22384146) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 23°C
Sample Mouse Cell (Brain)
Specification Brain
Permeabilization Yes - 0.5% Triton X 100 in PBS
Fixative Paraformaldehyde
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Abcam user community

Verified customer

提交于 Dec 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (brain tissue lysate, membrane fraction)
Loading amount 50 µg
Specification brain tissue lysate, membrane fraction
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Username

Abcam user community

Verified customer

提交于 Apr 12 2012

Thank you for your reply.
Reviewing the remaining antibodies that are already tested in WB and the species you wish to use, the following do not yet have a western blot image, and I would be pleased to arrange a testing discount for these addition...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"