Anti-Musashi 1 / Msi1抗体(ab21628)

概述

  • 产品名称Anti-Musashi 1 / Msi1抗体
    参阅全部 Musashi 1 / Msi1 一抗
  • 描述
    兔多克隆抗体to Musashi 1 / Msi1
  • 特异性Several customers have found that this antibody gives good results in rat however in our hands, we cannot obtain positive results. This antibody is therefore no longer covered by our Abpromise guarantee for use in rat.
  • 经测试应用适用于: ICC/IF, WBmore details
    不适用于: IHC-FoFr or IHC-P
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Musashi 1/ Msi1.

    (Peptide available as ab23870.)

  • 阳性对照
    • 46C Mouse neural precursor whole cell lysate (ab27205)

性能

应用

Our Abpromise guarantee covers the use of ab21628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).Can be blocked with Human Musashi 1 / Msi1 peptide (ab23870).
  • 应用说明Is unsuitable for IHC-FoFr or IHC-P.
  • 靶标

    • 功能RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
    • 组织特异性Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.
    • 序列相似性Belongs to the Musashi family.
      Contains 2 RRM (RNA recognition motif) domains.
    • 结构域The first RNA recognition motif binds more strongly to RNA compared to the second one.
    • 细胞定位Cytoplasm. Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Msi 1 antibody
      • Msi1 antibody
      • MSI1H_HUMAN antibody
      • Musashi homolog 1 antibody
      • Musashi-1 antibody
      • Musashi1 antibody
      • RNA binding protein Musashi homolog 1 antibody
      • RNA-binding protein Musashi homolog 1 antibody
      see all

    Anti-Musashi 1 / Msi1 antibody 图像

    • All lanes : Anti-Musashi 1 / Msi1 antibody (ab21628) at 1 µg/ml

      Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
      Lane 2 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205)
      Lane 3 : E14 Mouse Embryo Brain Tissue Lysate
      Lane 4 : E10 Mouse Embryo Brain Tissue Lysate
      Lane 5 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate with Human Musashi 1 / Msi1 peptide (ab23870) at 1 µg/ml
      Lane 6 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205) with Human Musashi 1 / Msi1 peptide (ab23870) at 1 µg/ml
      Lane 7 : E14 Mouse Embryo Brain Tissue Lysate with Human Musashi 1 / Msi1 peptide (ab23870) at 1 µg/ml
      Lane 8 : E10 Mouse Embryo Brain Tissue Lysate with Human Musashi 1 / Msi1 peptide (ab23870) at 1 µg/ml

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Predicted band size : 39 kDa
      Observed band size : 39 kDa
      Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.
      ab21628 recognized a band corresponding to the expected size of Musashi1/Msi1. The band was blocked using the immunising peptide (ab23870).
    • ab21628 at 1ug/ml staining human eye (limbal region) by ICC/IF. The cells were paraformaldehyde fixed and blocked with 2% BSA prior to incubation with antibody for 1 hour. A FITC conjugated sheep polyclonal antibody was used as the secondary.

      See Abreview

    • ICC/IF image of ab21628 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21628, 10µg/ml) overnight at +4°C. The secondary antibody (green) was a96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) PC12 cells at 10µg/ml.

    Anti-Musashi 1 / Msi1 antibody (ab21628)参考文献

    This product has been referenced in:
    • Zhang L  et al. CXCR4 activation promotes differentiation of human embryonic stem cells to neural stem cells. Neuroscience N/A:N/A (2016). ICC/IF ; Human . Read more (PubMed: 27615032) »
    • ErLin S  et al. Musashi-1 maintains blood-testis barrier structure during spermatogenesis and regulates stress granule formation upon heat stress. Mol Biol Cell 26:1947-56 (2015). Mouse . Read more (PubMed: 25717188) »

    See all 12 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Human iPSCs derived neural stem cell)
    Permeabilization Yes - 0.1% Triton X-100 in PBS
    Specification Human iPSCs derived neural stem cell
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Aug 03 2015

    Thank you very much for your interest our products.

    To our knowledge, neither ab21628 nor ab52865 have been tested in Xenopus. The homology of the immunogen of ab21628 with Xenopus laevis and tropicalis is 76%. The homology of the immunogen o...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Rat Tissue sections (Brain)
    Specification Brain
    Fixative Paraformaldehyde
    Antigen retrieval step None
    Permeabilization No
    Username

    Dr. Sophie Pezet

    Verified customer

    提交于 Sep 21 2012

    I would recommend ab52865, as it guaranteed to work in IHC-P, and will react with mouse, rat, human and and chicken. If there is anything else I can do, please let me know.

    The anti-Musashi 1 / Msi1 antibody, ab21628, was deemed to be unsuitable for IHC-P due to feedback we received from the human protein atlas, who said that it only gave non-specific staining. They did not provide the protocol they used to test the antib...

    Read More
    Application Immunohistochemistry (Frozen sections)
    Sample Apteronotus leptorhynchus Tissue sections (Intact cerebellum)
    Specification Intact cerebellum
    Fixative Formaldehyde
    Permeabilization Yes - 0.3% Triton X-100
    Blocking step 3% sheep serum, 1% BSA, 1% teleostean gelatine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Username

    Dr. Ruxandra Sirbulescu

    Verified customer

    提交于 Sep 28 2011

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Flow Cytometry
    Sample Human Cell (Neural stem cells derived from human embryonic ste)
    Specification Neural stem cells derived from human embryonic ste
    Preparation Cell harvesting/tissue preparation method: accutase digested single cell suspension from tissue culture
    Sample buffer: PBS
    Fixation BD Fixation/Permeabilization solution (catalog # 554714)
    Permeabilization Yes - BD Perm/Wash solution (catalog #554714)
    Gating Strategy live cells
    Username

    Dr. Jennifer Moore

    Verified customer

    提交于 Sep 06 2010

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (Brain sections)
    Specification Brain sections
    Fixative Paraformaldehyde
    Antigen retrieval step None
    Permeabilization No
    Username

    Dr. Sophie Pezet

    Verified customer

    提交于 Jun 17 2010

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (adult brain svz of lateral ventricle)
    Specification adult brain svz of lateral ventricle
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citric acid pH6
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Username

    Mr. Carl Hobbs

    Verified customer

    提交于 Oct 31 2007

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Human Limbal Region in Eyes)
    Specification Human Limbal Region in Eyes
    Fixative Paraformaldehyde
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2
    Username

    Miss. Ashley Quintana

    Verified customer

    提交于 Nov 30 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"