Anti-Mus81抗体[MTA30 2G10/3] (ab14387)

概述

  • 产品名称Anti-Mus81抗体[MTA30 2G10/3]
    参阅全部 Mus81 一抗
  • 描述
    小鼠单克隆抗体[MTA30 2G10/3] to Mus81
  • 经测试应用适用于: ICC/IF, WB, IP, Flow Cytmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse
  • 免疫原

    Mus81 protein.

性能

应用

Our Abpromise guarantee covers the use of ab14387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 23144634
WB 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 66 kDa). Tested on both recombinant protein and whole cell extract.
IP Use at an assay dependent concentration.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.
  • 组织特异性Widely expressed.
  • 序列相似性Belongs to the XPF family.
    Contains 1 ERCC4 domain.
  • 发展阶段Expressed in S phase and G2 phase.
  • 细胞定位Nucleus > nucleolus. Recruited to foci of DNA damage in S-phase cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Crossover junction endonuclease MUS81 antibody
    • FLJ21012 antibody
    • FLJ44872 antibody
    • Mus 81 antibody
    • MUS81 antibody
    • MUS81 Endonuclease Homolog antibody
    • MUS81_HUMAN antibody
    see all

Anti-Mus81 antibody [MTA30 2G10/3] 图像

  • Anti-Mus81 antibody [MTA30 2G10/3] (ab14387) at 1/1000 dilution + HeLa cell lysate

    Predicted band size : 66 kDa
    Observed band size : 64 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing HeLa cells stained with ab14387 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14387, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)参考文献

This product has been referenced in:
  • Thangavel S  et al. DNA2 drives processing and restart of reversed replication forks in human cells. J Cell Biol 208:545-62 (2015). Read more (PubMed: 25733713) »
  • Pepe A & West SC Substrate specificity of the MUS81-EME2 structure selective endonuclease. Nucleic Acids Res 42:3833-45 (2014). WB, ICC/IF ; Human . Read more (PubMed: 24371268) »

See all 15 Publications for this product

Product Wall

Application Western blot
Loading amount 5 µg
Gel Running Conditions Reduced Denaturing (8%)
Sample Human Cell lysate - whole cell (U2OS)
Specification U2OS
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Remi Buisson

Verified customer

提交于 Oct 31 2014

Application Immunoprecipitation
Immuno-precipitation step Protein G
Sample Human Cell lysate - whole cell (HEK293)
Specification HEK293
Total protein in input 1000 µg
Username

Abcam user community

Verified customer

提交于 Jun 20 2014

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (HEK293)
Specification HEK293
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

提交于 Jun 10 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (HeLa)
Specification HeLa
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Nov 05 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Loading amount 30 µg
Specification Mouse embryonic fibroblasts
Gel Running Conditions Reduced Denaturing
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Dec 13 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HeLa cells)
Total protein in input 200 µg
Specification HeLa cells
Immuno-precipitation step Protein A/G
Username

Abcam user community

Verified customer

提交于 Apr 06 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HCT116 p53wt cells)
Loading amount 30 µg
Specification HCT116 p53wt cells
Gel Running Conditions Reduced Denaturing (4-20% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Nov 04 2010

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (GM00637 human fibroblasts)
Specification GM00637 human fibroblasts
Fixative Paraformaldehyde
Permeabilization Yes - 0.4% Triton X-100
Blocking step FCS as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 31 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Expression hMus81 in Baculovirus system)
Loading amount 10000 cells
Specification Expression hMus81 in Baculovirus system
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Olga Mazin

Verified customer

提交于 Feb 12 2007

Thank you for your enquiry. Yes, ab14387 has only so far been tested for cross-reactivity with human Mus81 and we don't know how it will work with other species. I really can't say whether or not it will detect mouse Mus81. You might want to check to s...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"