概述

  • 产品名称Anti-MURF2抗体
    参阅全部 MURF2 一抗
  • 描述
    山羊多克隆抗体to MURF2
  • 经测试应用适用于: WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Dog, Pig
  • 免疫原

    Synthetic peptide:

    HIFSFSWLNSLNE

    , corresponding to C terminal amino acids 536-546 of Human MURF2.

性能

应用

Our Abpromise guarantee covers the use of ab4387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/0.1 - 1/0.3. Detects a band of approximately 85 kDa (predicted molecular weight: 60 kDa). PubMed: 17272810
IHC-Fr Use at an assay dependent dilution.

靶标

  • 功能May regulate gene expression and protein turnover in muscle cells.
  • 组织特异性Highly expressed in muscle. Low-level expression in liver.
  • 序列相似性Contains 1 B box-type zinc finger.
    Contains 1 COS domain.
    Contains 1 RING-type zinc finger.
  • 细胞定位Cytoplasm. Nucleus. Nuclear under atrophic conditions and upon mechanical signals. Localizes to the sarcomeric M-band in cardiomyocytes. Colocalizes in part with microtubules.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MURF 2 antibody
    • MURF-2 antibody
    • MuRF2 antibody
    • Muscle specific ring finger 2 antibody
    • Muscle specific RING finger protein 2 antibody
    • Muscle-specific RING finger protein 2 antibody
    • RING finger protein 29 antibody
    • RNF 29 antibody
    • RNF29 antibody
    • TRI55_HUMAN antibody
    • TRIM 55 antibody
    • Trim55 antibody
    • Tripartite motif containing 55 antibody
    • Tripartite motif containing protein 55 antibody
    • Tripartite motif-containing protein 55 antibody
    see all

Anti-MURF2 antibody 图像

  • Anti-MURF2 antibody (ab4387) at 0.1 µg/ml + Human heart lysate (in RIPA buffer) at 35 µg

    Predicted band size : 60 kDa

Anti-MURF2 antibody (ab4387)参考文献

This product has been referenced in:
  • Perera S  et al. Developmental regulation of MURF ubiquitin ligases and autophagy proteins nbr1, p62/SQSTM1 and LC3 during cardiac myofibril assembly and turnover. Dev Biol 351:46-61 (2011). WB ; Mouse . Read more (PubMed: 21185285) »
  • Willis MS  et al. Muscle ring finger 1, but not muscle ring finger 2, regulates cardiac hypertrophy in vivo. Circ Res 100:456-9 (2007). WB ; Mouse . Read more (PubMed: 17272810) »

See all 2 Publications for this product

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Muscle cell)
Gel Running Conditions Reduced Denaturing (12%)
Loading amount 60 µg
Specification Muscle cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Mr. Joao de Oliveira Silvestre

Verified customer

提交于 Feb 11 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (lung)
Specification lung
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Dec 18 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"