概述

  • 产品名称
  • 描述
    兔多克隆抗体to MURF1
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow
  • 免疫原

    Synthetic peptide containing a sequence corresponding to a region within amino acids 1-14 (

    MDYKSSLIQDGNPM

    ) of Human MURF1 (NP_115977).

  • 阳性对照
    • HepG2 whole cell lysate; HeLa cells; 293T, A431, H1299, HeLaS3, Molt-4 and Raji cell lines

性能

应用

Our Abpromise guarantee covers the use of ab96857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 40 kDa.
ICC/IF 1/100 - 1/200.

靶标

  • 功能
    E3 ubiquitin ligase. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells.
  • 组织特异性
    Muscle specific. Selectively expressed in heart and skeletal muscle. Also expressed in the iris.
  • 通路
    Protein modification; protein ubiquitination.
  • 序列相似性
    Contains 1 B box-type zinc finger.
    Contains 1 COS domain.
    Contains 1 RING-type zinc finger.
  • 发展阶段
    Expressed throughout all developmental stages.
  • 结构域
    The RING-type zinc finger mediates interaction with SUMO2 and localization to the nucleus. Also required for the E3 ubiquitin ligase activity.
  • 细胞定位
    Cytoplasm. Nucleus. Localizes to the M- and Z-lines in skeletal muscle. Colocalizes with TNNI3 in myocytes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • E3 ubiquitin-protein ligase TRIM63 antibody
    • FLJ32380 antibody
    • IRF antibody
    • Iris RING finger protein antibody
    • MURF 1 antibody
    • MURF-1 antibody
    • MuRF1 antibody
    • MURF2 antibody
    • Muscle specific ring finger protein 1 antibody
    • Muscle specific ring finger protein 2 antibody
    • Muscle-specific RING finger protein 1 antibody
    • OTTHUMP00000008701 antibody
    • RING finger protein 28 antibody
    • RNF 28 antibody
    • RNF28 antibody
    • SMRZ antibody
    • Striated muscle RING zinc finger protein antibody
    • TRI63_HUMAN antibody
    • TRIM 63 antibody
    • Trim63 antibody
    • Tripartite motif containing 63 antibody
    • tripartite motif containing 63, E3 ubiquitin protein ligase antibody
    • Tripartite motif containing protein 63 antibody
    • Tripartite motif-containing protein 63 antibody
    • Ubiquitin ligase TRIM63 antibody
    see all

图片

  • Anti-MURF1 antibody (ab96857) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 40 kDa
  • Immunofluorescence analysis of MURF1 in paraformaldehyde-fixed HeLa cells, using ab96857 at 1/200 dilution. Lower image is merged with DNA probe.

文献

This product has been referenced in:
  • MacNeil LG  et al. The order of exercise during concurrent training for rehabilitation does not alter acute genetic expression, mitochondrial enzyme activity or improvements in muscle function. PLoS One 9:e109189 (2014). WB ; Human . Read more (PubMed: 25289940) »
  • Bailey AN  et al. MAFbx, MuRF1, and the stress-activated protein kinases are upregulated in muscle cells during total knee arthroplasty. Am J Physiol Regul Integr Comp Physiol 303:R376-86 (2012). Human . Read more (PubMed: 22761181) »

See all 2 Publications for this product

客户评价及客户问答

Application
Western blot
Sample
Sheep Tissue lysate - whole (skeletal muscle)
Gel Running Conditions
Non-reduced Denaturing (10)
Loading amount
50 µg
Specification
skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Sep 23 2017

Thank you for contacting us.
Human Protein Reference Database shows that Murf1 may be expressed in liver tissue.
Please refer to:

http://www.hprd.org/summary?hprd_id=05843&isoform_id=05843_1&isoform_name=Isoform_1
Also,...

Read More
Application
Western blot
Sample
Pig Tissue lysate - other (Porcine skeletal muscle (longissimus dorsi))
Loading amount
30 µg
Specification
Porcine skeletal muscle (longissimus dorsi)
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
StartingBlock (TBS) Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jan 19 2012

I am very pleased to hear you would like to accept our offer and test ab96857 in swine. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forswine and include this code in the &ldq...

Read More

Thank you for your interest in Abcam's MuRF1 antibodies. I have performed a search of our catalog and specifically looked at ab77577 and ab96857 for suitability with swine. Unfortunately ab77577 is out of stock long term, however I agree that ab96857 w...

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Tibialis muscle of C57BL/6 and MuRF1/2 knock-out m)
Loading amount
5 µg
Specification
Tibialis muscle of C57BL/6 and MuRF1/2 knock-out m
Treatment
LPS or NaCl as a control 48h
Gel Running Conditions
Reduced Denaturing (4-12% bis-tris XT-Criterion BioRad)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Aug 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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