概述

  • 产品名称Anti-MURF1抗体
    参阅全部 MURF1 一抗
  • 描述
    小鼠单克隆抗体to MURF1
  • 经测试应用适用于: WB, Flow Cyt, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: DKSTKLVETA IQSLDEPGGA TFLLTAKQLI KSIVEASKGC QLGKTEQGFE NMDFFTLDLE HIADALRAID FGTDEEEEEF IEEEDQEEEE STEGKEEGH, corresponding to amino acids 254-353 of Human MURF1

性能

应用

Our Abpromise guarantee covers the use of ab57865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能E3 ubiquitin ligase. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells.
  • 组织特异性Muscle specific. Selectively expressed in heart and skeletal muscle. Also expressed in the iris.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Contains 1 B box-type zinc finger.
    Contains 1 COS domain.
    Contains 1 RING-type zinc finger.
  • 发展阶段Expressed throughout all developmental stages.
  • 结构域The RING-type zinc finger mediates interaction with SUMO2 and localization to the nucleus. Also required for the E3 ubiquitin ligase activity.
  • 细胞定位Cytoplasm. Nucleus. Localizes to the M- and Z-lines in skeletal muscle. Colocalizes with TNNI3 in myocytes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • E3 ubiquitin-protein ligase TRIM63 antibody
    • FLJ32380 antibody
    • IRF antibody
    • Iris RING finger protein antibody
    • MURF 1 antibody
    • MURF-1 antibody
    • MuRF1 antibody
    • MURF2 antibody
    • Muscle specific ring finger protein 1 antibody
    • Muscle specific ring finger protein 2 antibody
    • Muscle-specific RING finger protein 1 antibody
    • OTTHUMP00000008701 antibody
    • RING finger protein 28 antibody
    • RNF 28 antibody
    • RNF28 antibody
    • SMRZ antibody
    • Striated muscle RING zinc finger protein antibody
    • TRI63_HUMAN antibody
    • TRIM 63 antibody
    • Trim63 antibody
    • Tripartite motif containing 63 antibody
    • tripartite motif containing 63, E3 ubiquitin protein ligase antibody
    • Tripartite motif containing protein 63 antibody
    • Tripartite motif-containing protein 63 antibody
    • Ubiquitin ligase TRIM63 antibody
    see all

Anti-MURF1 antibody 图像

  • Western blot against tagged recombinant protein immunogen using ab57865 MURF1 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
  • IHC image of ab57865 staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab57865, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing HeLa cells stained with ab57865 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57865, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-MURF1 antibody (ab57865)参考文献

This product has been referenced in:
  • Salanova M  et al. Disuse deterioration of human skeletal muscle challenged by resistive exercise superimposed with vibration: evidence from structural and proteomic analysis. FASEB J 28:4748-63 (2014). Read more (PubMed: 25122557) »

See 1 Publication for this product

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Skeletal Muscle Cells, C2C12)
Loading amount 30 µg
Specification Skeletal Muscle Cells, C2C12
Treatment 1 uM DMSO for 24 hours
Gel Running Conditions Reduced Non-Denaturing (Native) (10% Polyacrylamide Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Oct 12 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"