Anti-MURC抗体(ab121644)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to MURC
  • 经测试应用
    适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen, corresponding to amino acids 1-90 (MEHNGSASNA DKIHQNRLSS VTEDEDQDAA LTIVTVLDKV ASIVDSVQAS QKRIEERHRE MENAIKSVQI DLLKLSQSHS NTGHIINKLF) of Human MURC.

  • 阳性对照
    • Human lymph node tissue.

性能

应用

Our Abpromise guarantee covers the use of ab121644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Induces RHOA activation and activates NPPA transcription and myofibrillar organization through the Rho/ROCK signaling pathway.
  • 序列相似性
    Belongs to the PTRF/SDPR family.
  • 细胞定位
    Cytoplasm > myofibril > sarcomere. Cytoplasm. In cardiomyocytes, accumulates in the Z-line of the sarcomere. In vascular smooth muscle cells, detected diffusely throughout the cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • murC antibody
    • MURC_HUMAN antibody
    • Muscle-related coiled-coil protein antibody
    • Muscle-restricted coiled-coil protein antibody

图片

  • ab121644, at 1/60 dilution, staining MURC in paraffin-embedded Human lymph node tissue, by Immunohistochemistry,
  • Immunohistochemical staining of Human skeletal muscle shows distinct membranous positivity using ab121644

文献

ab121644 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
10 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Feb 15 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (5 micron thick mouse heart tissue)
Permeabilization
Yes - 0.2% Triton X-100
Specification
5 micron thick mouse heart tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 30 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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