重组Anti-Munc 13-4抗体[EPR4914] (ab109113)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4914] to Munc 13-4
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-Munc 13-4抗体[EPR4914]
参阅全部 Munc 13-4 一抗 -
描述
兔单克隆抗体[EPR4914] to Munc 13-4 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: A673, K562, HepG2, Molt-4, and RAW264.7. Flow Cyt (intra): Molt-4.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4914 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109113于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 123 kDa.
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说明 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 123 kDa. |
靶标
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功能
Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. Regulates assembly of recycling and late endosomal structures, leading to the formation of an endosomal exocytic compartment that fuses with perforin-containing granules at the immunologic synapse and licences them for exocytosis. Regulates Ca(2+)-dependent secretory lysosome exocytosis in mast cells. -
组织特异性
Expressed at high levels in spleen, thymus and leukocytes. Also expressed in lung and placenta, and at very low levels in brain, heart, skeletal muscle and kidney. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells. -
疾病相关
Defects in UNC13D are the cause of hemophagocytic lymphohistiocytosis familial type 3 (FHL3) [MIM:608898]; also known as HPLH3. Familial hemophagocytic lymphohistiocytosis (FHL) is a genetically heterogeneous, rare autosomal recessive disorder. It is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, and neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits, and ataxia. Hemophagocytosis is a prominent feature of the disease, and a non-malignant infiltration of macrophages and activated T lymphocytes in lymph nodes, spleen, and other organs is also found. -
序列相似性
Belongs to the unc-13 family.
Contains 2 C2 domains.
Contains 1 MHD1 (MUNC13 homology domain 1) domain.
Contains 1 MHD2 (MUNC13 homology domain 2) domain. -
结构域
The MHD1 and MHD2 domains mediate localization on recycling endosomes and lysosome. -
细胞定位
Cytoplasm. Membrane. Late endosome. Recycling endosome. Lysosome. Colocalizes with cytotoxic granules at the plasma membrane. Localizes to endosomal exocytic vesicles. - Information by UniProt
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数据库链接
- Entrez Gene: 201294 Human
- Entrez Gene: 70450 Mouse
- Omim: 608897 Human
- SwissProt: Q70J99 Human
- SwissProt: B2RUP2 Mouse
- Unigene: 41045 Human
- Unigene: 259460 Mouse
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形式
There are 3 isoforms produced by alternative splicing. -
别名
- FHL 3 antibody
- FHL3 antibody
- FLJ00067 antibody
see all
图片
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All lanes : Anti-Munc 13-4 antibody [EPR4914] (ab109113) at 1/1000 dilution
Lane 1 : A673 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Molt-4 cell lysate
Lane 5 : RAW264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 123 kDa -
Intracellular flow cytometric analysis of permeabilized Molt-4 cells using ab109113 in red.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab109113 被引用在 3 文献中.
- Takushi SE et al. Lentiviral Gene Therapy for Familial Hemophagocytic Lymphohistiocytosis Type 3, Caused by UNC13D Genetic Defects. Hum Gene Ther 31:626-638 (2020). PubMed: 32253931
- Krawczynski K et al. Unc-13 homolog D mediates an antiviral effect of the chromosome 19 microRNA cluster miR-517a. J Cell Sci 134:N/A (2020). PubMed: 33093239
- Xu R et al. Munc13-4 mediates human neutrophil elastase-induced airway mucin5AC hypersecretion by interacting with syntaxin2. Mol Med Rep 18:1015-1024 (2018). PubMed: 29767240