概述

  • 产品名称Anti-Munc 13-4抗体
    参阅全部 Munc 13-4 一抗
  • 描述
    山羊多克隆抗体to Munc 13-4
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Dog, Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide:

    C-ETQKHKKDLHPLFD

    , corresponding to amino acids 967-980 of Human Munc 13-4 (NP_954712.1)

  • 阳性对照
    • Human Spleen and Human Tonsil lysates; Human Thymus tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab77057 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab77057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
IHC-P
  • 应用说明ELISA: Use at an assay dependent dilution. Peptide ELISA antibody detection limit dilution: 1/128000
    IHC-P: Use at a concentration of 2.5 µg/ml.
    WB: Use at a concentration of 0.1 µg/ml. Predicted molecular weight: 123 kDa.
    Preliminary experiments gave an approx 75kDa band in Human Spleen and Human Tonsil lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 123kDa according to NP_954712.1 Other isoforms have been described in other species. The band was successfully blocked by incubation with the immunising peptide


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. Regulates assembly of recycling and late endosomal structures, leading to the formation of an endosomal exocytic compartment that fuses with perforin-containing granules at the immunologic synapse and licences them for exocytosis. Regulates Ca(2+)-dependent secretory lysosome exocytosis in mast cells.
    • 组织特异性Expressed at high levels in spleen, thymus and leukocytes. Also expressed in lung and placenta, and at very low levels in brain, heart, skeletal muscle and kidney. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells.
    • 疾病相关Defects in UNC13D are the cause of hemophagocytic lymphohistiocytosis familial type 3 (FHL3) [MIM:608898]; also known as HPLH3. Familial hemophagocytic lymphohistiocytosis (FHL) is a genetically heterogeneous, rare autosomal recessive disorder. It is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, and neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits, and ataxia. Hemophagocytosis is a prominent feature of the disease, and a non-malignant infiltration of macrophages and activated T lymphocytes in lymph nodes, spleen, and other organs is also found.
    • 序列相似性Belongs to the unc-13 family.
      Contains 2 C2 domains.
      Contains 1 MHD1 (MUNC13 homology domain 1) domain.
      Contains 1 MHD2 (MUNC13 homology domain 2) domain.
    • 结构域The MHD1 and MHD2 domains mediate localization on recycling endosomes and lysosome.
    • 细胞定位Cytoplasm. Membrane. Late endosome. Recycling endosome. Lysosome. Colocalizes with cytotoxic granules at the plasma membrane. Localizes to endosomal exocytic vesicles.
    • Information by UniProt
    • 数据库链接
    • 形式There are 3 isoforms produced by alternative splicing.
    • 别名
      • FHL 3 antibody
      • FHL3 antibody
      • FLJ00067 antibody
      • HLH 3 antibody
      • HLH3 antibody
      • HPLH 3 antibody
      • HPLH3 antibody
      • Jinx antibody
      • Munc13 4 antibody
      • Munc13-4 antibody
      • Protein unc 13 homolog D antibody
      • Protein unc-13 homolog D antibody
      • UN13D_HUMAN antibody
      • Unc 13 homolog D antibody
      • UNC 13D antibody
      • Unc-13 homolog D (C. elegans) antibody
      • Unc13 homolog D (C elegans) antibody
      • Unc13 homolog D antibody
      • UNC13, C. elegans, homolog of, D antibody
      • UNC13D antibody
      • Unc13h4 antibody
      see all

    Anti-Munc 13-4 antibody 图像

    • ab77057, at 2.5µg/ml, staining Munc 13-4 in paraffin-embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

    Anti-Munc 13-4 antibody (ab77057)参考文献

    ab77057 has not yet been referenced specifically in any publications.

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