重组
RabMAb

Anti-MUM1抗体[EPR5653] (ab124691)

概述

  • 产品名称
    Anti-MUM1抗体[EPR5653]
    参阅全部 MUM1 一抗
  • 描述
    兔单克隆抗体[EPR5653] to MUM1
  • 特异性
    Uniprot ID - Q15306
  • 经测试应用
    适用于: WB, IP, IHC-Pmore details
    不适用于: Flow Cyt or ICC
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide, corresponding to residues from the N-terminus of Human MUM1.

  • 阳性对照
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab124691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 应用说明
    Is unsuitable for Flow Cyt or ICC.
  • 靶标

    • 功能
      Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.
    • 组织特异性
      Lymphoid cells.
    • 疾病相关
      Defects in IRF4 are a cause of multiple myeloma (MM) [MIM:254500]. MM is a malignant tumor of plasma cells usually arising in the bone marrow and characterized by diffuse involvement of the skeletal system, hyperglobulinemia, Bence-Jones proteinuria and anemia. Complications of multiple myeloma are bone pain, hypercalcemia, renal failure and spinal cord compression. The aberrant antibodies that are produced lead to impaired humoral immunity and patients have a high prevalence of infection. Amyloidosis may develop in some patients. Multiple myeloma is part of a spectrum of diseases ranging from monoclonal gammopathy of unknown significance (MGUS) to plasma cell leukemia. Note=A chromosomal aberration involving IRF4 is found in multiple myeloma. Translocation t(6;14)(p25;q32) with the IgH locus.
    • 序列相似性
      Belongs to the IRF family.
      Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Interferon regulatory factor 4 antibody
      • IRF 4 antibody
      • IRF-4 antibody
      • Irf4 antibody
      • IRF4_HUMAN antibody
      • LSIRF antibody
      • Lymphocyte specific interferon regulatory factor antibody
      • Lymphocyte specific IRF antibody
      • Lymphocyte-specific interferon regulatory factor antibody
      • Multiple myeloma oncogene 1 antibody
      • MUM 1 antibody
      • MUM1 antibody
      • NF EM5 antibody
      • NF-EM5 antibody
      • NFEM5 antibody
      • PU.1 interaction partner antibody
      • Sfpi1/PU.1 interaction partner antibody
      • Transcriptional activator PIP antibody
      see all

    图片

    • All lanes : Anti-MUM1 antibody [EPR5653] (ab124691) at 1/1000 dilution

      Lane 1 : Ramos cell lysate
      Lane 2 : HuT 78 cell lysate
      Lane 3 : IM 9 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 52 kDa
    • ab124691, at 1/100, staining MUM1 in paraffin-embedded Human colon tissue by Immunohistochemistry.
    • ab124691, at 1/100, staining MUM1 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
    • Immunohistochemical analysis of dog spleen tissue, staining MUM1 with ab124691.

      Tissue was fixed with formaldehyde and blocked with 2.5% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a TRIS-EDTA buffer (pH 9). Samples were incubated with primary antibody (1/50 in PBS plus 1X casein) for 1 hour 30 minutes at 37°C. An undiluted HRP-conjugated horse anti-rabbit polyclonal IgG was used as the secondary antibody.

      See Abreview

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    文献

    This product has been referenced in:
    • Vieyra-Garcia PA  et al. STAT3/5-Dependent IL9 Overexpression Contributes to Neoplastic Cell Survival in Mycosis Fungoides. Clin Cancer Res 22:3328-39 (2016). IHC-P ; Human . Read more (PubMed: 26851186) »

    See 1 Publication for this product

    客户评价及客户问答

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (spleen)
    Specification
    spleen
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA (pH 9.0)
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Username

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    提交于 Nov 30 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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