重组Alexa Fluor® 555荧光Anti-Mucin 5AC抗体[EPR16904] (ab218714)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 555 Rabbit monoclonal [EPR16904] to Mucin 5AC
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm
Related conjugates and formulations
概述
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产品名称
Alexa Fluor® 555荧光Anti-Mucin 5AC抗体[EPR16904]
参阅全部 Mucin 5AC 一抗 -
描述
Alexa Fluor® 555荧光兔单克隆抗体[EPR16904] to Mucin 5AC -
宿主
Rabbit -
偶联物
Alexa Fluor® 555. Ex: 555nm, Em: 565nm -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment within Human Mucin 5AC aa 2300-2500. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P98088 -
阳性对照
- ICC/IF: HeLa cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR16904 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab218714于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | (1) |
1/50.
This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min) |
说明 |
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ICC/IF
1/50. This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min) |
靶标
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功能
Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system. -
组织特异性
Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum. -
序列相似性
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 3 TIL (trypsin inhibitory-like) domains.
Contains 4 VWFC domains.
Contains 4 VWFD domains. -
结构域
The cysteine residues in the Cys-rich subdomain repeats are not involved in disulfide bonding. -
翻译后修饰
C-, O- and N-glycosylated. O-glycosylated on the Thr-/Ser-rich tandem repeats. C-mannosylation in the Cys-rich subdomains may be required for proper folding of these regions and for export from the endoplasmic reticulum during biosynthesis.
Proteolytic cleavage in the C-terminal is initiated early in the secretory pathway and does not involve a serine protease. The extent of cleavage is increased in the acidic parts of the secretory pathway. Cleavage generates a reactive group which could link the protein to a primary amide. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 4586 Human
- Omim: 158373 Human
- SwissProt: P98088 Human
- Unigene: 534332 Human
- Unigene: 558950 Human
- Unigene: 703588 Human
- Unigene: 703728 Human
- Unigene: 721515 Human
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别名
- Apomucin antibody
- Apomucin Major Airway Glycoprotein antibody
- Gastric mucin antibody
see all
图片
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ab218714 staining Mucin 5AC in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab218714 at 1/50 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab218714 被引用在 5 文献中.
- Stölting H et al. Distinct airway epithelial immune responses after infection with SARS-CoV-2 compared to H1N1. Mucosal Immunol 15:952-963 (2022). PubMed: 35840680
- Marescotti D et al. Development of an Advanced Multicellular Intestinal Model for Assessing Immunomodulatory Properties of Anti-Inflammatory Compounds. Front Pharmacol 12:639716 (2021). PubMed: 33935729
- Luvanda MK et al. Dexamethasone Creates a Suppressive Microenvironment and Promotes Aspergillus fumigatus Invasion in a Human 3D Epithelial/Immune Respiratory Model. J Fungi (Basel) 7:N/A (2021). PubMed: 33803702
- Robinot R et al. SARS-CoV-2 infection induces the dedifferentiation of multiciliated cells and impairs mucociliary clearance. Nat Commun 12:4354 (2021). PubMed: 34272374
- Bovard D et al. Comparison of the basic morphology and function of 3D lung epithelial cultures derived from several donors. Curr Res Toxicol 1:56-69 (2020). PubMed: 34345837