Anti-Mucin 5AC抗体[CLH2] (ab77576)

概述

  • 产品名称Anti-Mucin 5AC抗体[CLH2]
    参阅全部 Mucin 5AC 一抗
  • 描述
    小鼠单克隆抗体[CLH2] to Mucin 5AC
  • 特异性ab77576 is specific for the MUC5AC tandem repeat of human mucin. No cross reactivity with other peptides from MUC2, 3, 6 or 7 The antibody recognises glycosylated as well as unglycosylated MUC5AC.
  • 经测试应用适用于: ICC/IF, WB, IP, IHC-P, IHC-Fr, Indirect ELISA, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide of the MUC5AC tandem repeat (Human).

  • 阳性对照
    • Native mucin extract from normal gastric mucosa and from gastric diffuse carcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab77576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB 1/100. Predicted molecular weight: 252 kDa.
IP Use at an assay dependent concentration.
IHC-P 1/1000 - 1/2000.
IHC-Fr 1/1000 - 1/2000.
Indirect ELISA Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 相关性Mucins are high molecular weight glycoproteins with 80% carbohydrates and 20% core protein. Gastric Mucin 5AC antigen is found in columnar mucus cells of surface gastric epithelium and in goblet cells of the fetal and precancerous colon but not in normal colon. Resurgence of gastric mucin during colonic carcinogenesis is suggestive of either re-expression of the peptide core of gastric mucin in the adult colon or due to changes in the glycosylation pattern of mucin, which expose the hidden Mucin 5AC antigen.
  • 细胞定位Secreted; Cytoplasmic and cell surface
  • 数据库链接
  • 别名
    • Apomucin antibody
    • Apomucin Major Airway Glycoprotein antibody
    • Gastric mucin antibody
    • LeB antibody
    • Lewis B blood group antigen antibody
    • Major Airway Glycoprotein antibody
    • MUC 5 antibody
    • MUC5 antibody
    • MUC5AC antibody
    • Mucin 5 subtype AC tracheobronchial antibody
    • Mucin 5 Subtypes A And C antibody
    • Mucin 5 subtypes A and C tracheobronchial/gastric antibody
    • Mucin 5, tracheobronchial/gastric antibody
    • Mucin 5AC antibody
    • Mucin 5AC oligomeric mucus/gel forming antibody
    • Mucin 5AC, oligomeric mucus/gel forming pseudogene antibody
    • TBM antibody
    • Tracheobronchial Mucin (TBM) antibody
    • Tracheobronchial mucin antibody
    see all

Anti-Mucin 5AC antibody [CLH2] 图像

  • ab77576 staining of foveolar epithelium of human gastric mucosa using IHC-Fr. Detection with a biotin-conjugated rabbit anti-mouse secondary, followed by avidin-biotin peroxidase complex. Colour Development by DAB. Hematoxylin counterstained.
  • ab77576 staining of foveolar epithelium of human gastric mucosa by IHC-Fr. Detection with a biotin-conjugated rabbit anti-mouse secondary, followed by avidin-biotin peroxidase complex.
  • Overlay histogram showing A549 cells stained with ab77576 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77576, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Mucin 5AC antibody [CLH2] (ab77576)参考文献

ab77576 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab77576.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"