概述

  • 产品名称Anti-Mucin 5AC抗体
    参阅全部 Mucin 5AC 一抗
  • 描述
    兔多克隆抗体to Mucin 5AC
  • 经测试应用适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 5000 to the C-terminus of Human Mucin 5AC.

  • 阳性对照
    • This antibody gave a positive signal in Human normal stomach formalin fixed paraffin embedded tissue section. This antibody gave a positive signal in the following formaldehyde fixed cell line: HeLa.

性能

应用

Our Abpromise guarantee covers the use of ab78660 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 相关性Mucins are high molecular weight glycoproteins with 80% carbohydrates and 20% core protein. Gastric Mucin 5AC antigen is found in columnar mucus cells of surface gastric epithelium and in goblet cells of the fetal and precancerous colon but not in normal colon. Resurgence of gastric mucin during colonic carcinogenesis is suggestive of either re-expression of the peptide core of gastric mucin in the adult colon or due to changes in the glycosylation pattern of mucin, which expose the hidden Mucin 5AC antigen.
  • 细胞定位Secreted; Cytoplasmic and cell surface
  • 数据库链接
  • 别名
    • Apomucin antibody
    • Apomucin Major Airway Glycoprotein antibody
    • Gastric mucin antibody
    • LeB antibody
    • Lewis B blood group antigen antibody
    • Major Airway Glycoprotein antibody
    • MUC 5 antibody
    • MUC5 antibody
    • MUC5AC antibody
    • Mucin 5 subtype AC tracheobronchial antibody
    • Mucin 5 Subtypes A And C antibody
    • Mucin 5 subtypes A and C tracheobronchial/gastric antibody
    • Mucin 5, tracheobronchial/gastric antibody
    • Mucin 5AC antibody
    • Mucin 5AC oligomeric mucus/gel forming antibody
    • Mucin 5AC, oligomeric mucus/gel forming pseudogene antibody
    • TBM antibody
    • Tracheobronchial Mucin (TBM) antibody
    • Tracheobronchial mucin antibody
    see all

Anti-Mucin 5AC antibody 图像

  • IHC image of Mucin 5AC staining in Human normal stomach formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78660, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • ICC/IF image of ab78660 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab78660 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Mucin 5AC antibody (ab78660)参考文献

This product has been referenced in:
  • Knoop KA  et al. Microbial sensing by goblet cells controls immune surveillance of luminal antigens in the colon. Mucosal Immunol N/A:N/A (2014). Read more (PubMed: 25005358) »

See 1 Publication for this product

Product Wall

Thank you for contacting us.

As indicated on the datasheet the antibody ab78660 does target secreted MUC5 in addition to targeting cytoplasmic MUC5 and MUC5 found on the cell surface.

We are happy to help customers find the most suita...

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Merci de votre intérêt pour l'anti-Mucin5AC ab78660 et le secondaire ab96899.

En effet, l'anticorps ab78660 n’a pas encore été testé chez le cheval et nous n’offrons pas d’échantillon ...

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Thank you for contacting us and your interest in our products. We have several antibodies directed towards the human Mucin 5AC protein. However, only one is raised in rabbits as you have stipulated.

This antibody is http://www.abcam.com/Mucin...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"