Anti-Mucin 5AC抗体[45M1] (ab3649)

概述

  • 产品名称Anti-Mucin 5AC抗体[45M1]
    参阅全部 Mucin 5AC 一抗
  • 描述
    小鼠单克隆抗体[45M1] to Mucin 5AC
  • 经测试应用适用于: Flow Cyt, ICC/IF, IHC-P, IP, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Tissue, cells or virus corresponding to Mucin 5AC. Prepared from the fluid of human ovarian mucinous cyst.

  • 阳性对照
    • Suitable tissue control sections for Immunohistochemistry: Stomach.

性能

应用

Our Abpromise guarantee covers the use of ab3649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration. PubMed: 20810726
IHC-P 1/100.
IP Use at an assay dependent concentration. PubMed: 17356062
ELISA 1/200. PubMed: 19755931

靶标

  • 相关性Mucins are high molecular weight glycoproteins with 80% carbohydrates and 20% core protein. Gastric Mucin 5AC antigen is found in columnar mucus cells of surface gastric epithelium and in goblet cells of the fetal and precancerous colon but not in normal colon. Resurgence of gastric mucin during colonic carcinogenesis is suggestive of either re-expression of the peptide core of gastric mucin in the adult colon or due to changes in the glycosylation pattern of mucin, which expose the hidden Mucin 5AC antigen.
  • 细胞定位Secreted; Cytoplasmic and cell surface
  • 数据库链接
  • 别名
    • Apomucin antibody
    • Apomucin Major Airway Glycoprotein antibody
    • Gastric mucin antibody
    • LeB antibody
    • Lewis B blood group antigen antibody
    • Major Airway Glycoprotein antibody
    • MUC 5 antibody
    • MUC5 antibody
    • MUC5AC antibody
    • Mucin 5 subtype AC tracheobronchial antibody
    • Mucin 5 Subtypes A And C antibody
    • Mucin 5 subtypes A and C tracheobronchial/gastric antibody
    • Mucin 5, tracheobronchial/gastric antibody
    • Mucin 5AC antibody
    • Mucin 5AC oligomeric mucus/gel forming antibody
    • Mucin 5AC, oligomeric mucus/gel forming pseudogene antibody
    • TBM antibody
    • Tracheobronchial Mucin (TBM) antibody
    • Tracheobronchial mucin antibody
    see all

Anti-Mucin 5AC antibody [45M1] 图像

  • ab3649 staining stomach by IHC-P.
  • Overlay histogram showing A549 cells stained with ab3649 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3649, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 80% methanol (5 min) used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • Immunohistochemical analysis of rat stomach tissue, staining Mucin 5AC with ab3649.

    Antigen retrieval was performed via heat mediation in a citrate buffer. Samples were blocked before incubating with primary antibody (1/100) for 1 hour at room temperature. An HRP-conjugated anti-mouse IgG was used as the secondary antibody and staining was detected using DAB.
  • Immunohistochemical analysis of human stomach tissue, staining Mucin 5AC with ab3649.

  • Immunohistochemical analysis of human stomach Adenocarcinoma, staining Mucin 5AC with ab3649

  • Immunohistochemical analysis of human colon Adenocarcinoma, staining Mucin 5AC with ab3649.

Anti-Mucin 5AC antibody [45M1] (ab3649)参考文献

This product has been referenced in:
  • Chiou SH  et al. Pancreatic cancer modeling using retrograde viral vector delivery and in vivo CRISPR/Cas9-mediated somatic genome editing. Genes Dev 29:1576-85 (2015). IHC-P ; Mouse . Read more (PubMed: 26178787) »
  • Sumer H  et al. Functional evaluation of ES-somatic cell hybrids in vitro and in vivo. Cell Reprogram 16:167-74 (2014). IHC-P . Read more (PubMed: 24787484) »

See all 17 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TE pH8
Permeabilization No
Specification lung
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative 10% normal buffered formalin
Username

Abcam user community

Verified customer

提交于 Aug 26 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (stomach)
Permeabilization No
Specification stomach
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Acetone
Username

Abcam user community

Verified customer

提交于 Aug 22 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (stomach)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TE pH8
Permeabilization No
Specification stomach
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative 10% buffered normal formalin
Username

Abcam user community

Verified customer

提交于 Aug 22 2016

Unfortunately, the specific epitope of ab3649 has not been mapped to our knowledge, so we do not know where within the Mucin 5AC protein the antibody binds.

Thank you for getting back to me and for providing some experimental details.

The protocol looks fine to me. It may well be that the antibody has been damaged during storage or shipment.

This antibody has not been tested with Mouse ...

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I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application us...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lung)
Specification Lung
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 May 15 2012

Thank you for your reply.

To clarify, the discount I offered is not a free sample, but can be used to get a free antibody on a future order after submitting an Abreview for ab79082.

This antibody was validated using formalin-fixed p...

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Yes, Sapphire Bioscienceis our distributor in Australia.

DISCOUNT CODE: ***
Expiration date: August 28th, 2012

I am very pleased to hear you would like to accept our offer and test ab79082 in canine. This code will give you: 1 ...

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Thank you for contacting us.

Ourantibodies to Mucin 5AC that have been validated for use on frozen tissues are primarily mouse monoclonal antibodies raised against the full length protein, for which the epitopes have not been mapped. Addition...

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