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Mucin isolated from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification) Human.
This antibody reacts with peptide core of gastric mucin restricted to the surface gastric epithelium. It characterised the M1-b epitope, completely destroyed by b-mercapto-ethano. The M1-b epitope is found located on a 58 kD and 40 kD peptide after trypsin treatment of native M1 mucin. This M1-b epitope coprecipites with the M1-f epitope encoded by Mucin 5AC gene. It is probably associated with the gastric mucin encoded by Mucin 5AC gene or with a gastric mucin encoded by another gastric MUC gene which polymerized with the M1/Mucin 5AC gastric mucin.
Our Abpromise guarantee covers the use of ab24071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||1/1000. PubMed: 19503797|
|Flow Cyt||Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|IHC-P||Use at an assay dependent concentration. The use of periodate solution (0.1 - 0.05M) for 10-20 minutes is recommended when detecting mucin by IHC.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 130 kDa.|
ab24071 at 1/1000 dilution staining MUcin 5Ac in Human bronchial epithelia by Immunocytochemistry. Well-differentiated HBE were exposed for 24 hr to mucosal PBS or mucosal SMM (Supernatant of mucopurulent material) before experiment. An immunoperoxidase detection system was used for staining.