Anti-MUC4抗体(ab76038)

概述

  • 产品名称Anti-MUC4抗体
    参阅全部 MUC4 一抗
  • 描述
    兔多克隆抗体to MUC4
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 2150 to the C-terminus of Human MUC4.

    (Peptide available as ab88229.)

  • 阳性对照
    • This antibody gave a positive signal in PC3 Whole Cell Lysate.

性能

应用

Our Abpromise guarantee covers the use of ab76038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 268 kDa (predicted molecular weight: 232 kDa).

靶标

  • 功能May play a role in tumor progression. Ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation. Has anti-adhesive properties. Seems to alter cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and in its ability to act as an intramembrane ligand for ERBB2. Plays an important role in cell proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. The MUC4-ERBB2 complex causes site-specific phosphorylation of the ERBB2 'Tyr-1248'. In polarized epithelilal cells segragates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation.
  • 组织特异性Expressed in the thymus, thyroid, lung, trachea, esophagus, stomach, small intestine, colon, testis, prostate, ovary, uterus, placenta, and mammary and salivary glands. Expressed in carcinomas arising from some of these epithelia, such as lung cancers, squamous cell carcinomas of the upper aerodigestive tract, mammary carcinomas, biliary tract, colon, and cervix cancers. Minimally or not expressed in the normal pancreas or chronic pancreatitis, but is highly expressed in pancreatic tumors and pancreatic tumor cell lines.
  • 序列相似性Contains 1 AMOP domain.
    Contains 2 EGF-like domains.
    Contains 1 NIDO domain.
    Contains 1 VWFD domain.
  • 发展阶段Expressed early in the primitive gut before respiratory and digestive epithelial cells have acquired their tissue and cell specificity. Expressed at the basal surface of the epithelium from week 14 to 26 weeks and then predominantly localized in only parietal cells. Immediately before birth, found in the cytoplasm of the mucous columnar epithelial cells. In the embryo expressed in skin, then disappears late in gestation.
  • 翻译后修饰Proteolytically cleaved into 2 chains, mucin-4 alpha chain and mucin-4 beta chain.
    mucrnin-4 alpha chain is highly O-glycosylated.
    mucin-4 beta chain is predominantly N-glycosylated.
  • 细胞定位Secreted; Cell membrane and Membrane. Secreted. Isoforms lacking the Cys-rich region, EGF-like domains and transmembrane region are secreted. Secretion occurs by splicing or proteolytic processing.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ascites sialoglycoprotein 1 antibody
    • Ascites sialoglycoprotein 2 antibody
    • Ascites sialoglycoprotein antibody
    • ASGP antibody
    • ASGP-1 antibody
    • ASGP-2 antibody
    • HSA276359 antibody
    • MUC 4 antibody
    • MUC-4 antibody
    • Muc4 antibody
    • MUC4_HUMAN antibody
    • Mucin 4 antibody
    • Mucin 4 cell surface associated antibody
    • Mucin 4 tracheobronchial antibody
    • Mucin-4 beta chain antibody
    • Pancreatic adenocarcinoma mucin antibody
    • Testis mucin antibody
    • Tracheobronchial mucin antibody
    • Tracheobronchial mucin Fragment antibody
    see all

Anti-MUC4 antibody 图像

  • Anti-MUC4 antibody (ab76038) at 1 µg/ml + PC3 (Human prostate carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 232 kDa
    Observed band size : 268 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa (possible isoform).

    Exposure time : 16 minutes
  • ICC/IF image of ab76038 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76038, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-MUC4 antibody (ab76038)参考文献

ab76038 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. Below is the link for the SwissProt page for MUC4 that the immunogen is taken from:

http://www.uniprot.org/uniprot/Q99102

The immunogen should detect isoforms 1,10,12,13,17. Isoform 1 is considered the dom...

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Thank you for your inquiry.

I am sorry to confirm that (as stated in yesterday's email CCE xxxxx) we do not have any MUC4 or MUC13 antibodies that are suitable for mouse samples.

We expect a cross reaction to another species of an...

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