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Our Abpromise guarantee covers the use of ab52263 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration. PubMed: 14729877|
|ICC/IF||Use at an assay dependent concentration. PubMed: 14729877|
|WB||Use at an assay dependent concentration. PubMed: 14729877|
|IHC-P||1/200 - 1/300.|
Formaldehyde fixed human urinary tract tissue stained for MUC4 with ab52263 (1/100 dilution) in immunohistochemical analysis.
IHC image of ab52263 staining in human pancreatic carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 minutes. The section was then incubated with ab52263, 5 µg/ml, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"