Anti-MUC2抗体[996/1] (ab11197)

概述

  • 产品名称
    Anti-MUC2抗体[996/1]
    参阅全部 MUC2 一抗
  • 描述
    小鼠单克隆抗体[996/1] to MUC2
  • 特异性
    ab11197 recognises the human MUC2 mucin, and shows no cross-reactivity with MUC1, MUC3 or MUC4 mucins. In tissue sections ab11197 recognises colon, liver and prostate tissues strongly. The antibody recognises malignant colonic mucosa as well as normal mucosa.
  • 经测试应用
    适用于: ICC/IF, Flow Cyt, IHC-Fr, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human MUC2. Synthetic peptide: TPTPTGTQTP TTTPITTTTT VTPTPTPTGT QTPTTTPITT T This is from the tandem repeat are of the sequence and can be found starting at amino acids 1933 and 2163
    Sequence:

    TPTPTGTQ TPTTTPITTT TTVTPTPTPT GTQTPTTTPI TTT


    Database link: Q02817

  • 阳性对照
    • Normal colon
  • 常规说明

    We have removed mouse from our list of reactive species based on contradictory results from researchers. We have had increased reports of negative staining results in IHC-P with mouse tissue. We do not batch test this antibody with mouse and we will no longer guarantee it for mouse reactivity.

     

    Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

性能

应用

Our Abpromise guarantee covers the use of ab11197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 20418870
Flow Cyt Use 10µl for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. This product does not require protein digestion or antigen retrieval using heat treatment prior to staining of paraffin sections.
WB Use at an assay dependent concentration. Predicted molecular weight: 621.5 kDa.

靶标

  • 功能
    Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.
  • 组织特异性
    Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder.
  • 序列相似性
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 TIL (trypsin inhibitory-like) domain.
    Contains 2 VWFC domains.
    Contains 4 VWFD domains.
  • 翻译后修饰
    O-glycosylated.
    May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.
    At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway.
  • 细胞定位
    Secreted. In the intestine, secreted into the inner and outer mucus layers.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Intestinal mucin 2 antibody
    • Intestinal mucin-2 antibody
    • MLP antibody
    • MUC 2 antibody
    • MUC-2 antibody
    • Muc2 antibody
    • MUC2_HUMAN antibody
    • Mucin 2 antibody
    • Mucin 2 intestinal antibody
    • Mucin 2 intestinal/tracheal antibody
    • Mucin 2 oligomeric mucus/gel forming antibody
    • Mucin 2 precursor antibody
    • Mucin 2 tracheal antibody
    • Mucin like protein antibody
    • Mucin-2 antibody
    • Mucin2 antibody
    • SMUC antibody
    see all

Anti-MUC2 antibody [996/1] (ab11197)参考文献

This product has been referenced in:
  • Jiang Y  et al. Expression of inositol-requiring enzyme 1ß is downregulated in colorectal cancer. Oncol Lett 13:1109-1118 (2017). IHC-P ; Human . Read more (PubMed: 28454221) »
  • Forbester JL  et al. Interaction of Salmonella enterica Serovar Typhimurium with Intestinal Organoids Derived from Human Induced Pluripotent Stem Cells. Infect Immun 83:2926-34 (2015). IHC . Read more (PubMed: 25964470) »

See all 11 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Tumor Tissue)
Permeabilization
Yes - 0.3% Triton
Specification
Tumor Tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Dr. Ashok kumar Dilly

Verified customer

提交于 Sep 20 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse Tissue Section (Colon))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6
Permeabilization
Yes - Triton 0.2%/PBS
Specification
Mouse Tissue Section (Colon)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative
Formaldehyde
Username

Ms. Claudia Champagne

Verified customer

提交于 Jun 22 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (peritonei tumor, Epithelial cell type)
Permeabilization
No
Specification
peritonei tumor, Epithelial cell type
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 07 2015

Thank you for your inquiry.

Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information.

We aim to provide as much information as possible to our customers, so I a...

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Thank you for contacting us.

According to our Technical FAQs, we would like to suggest to use 1 ug/ml as a starting concentration for this antibody.



Regarding the gel we would like suggest to use a gel between 5% and 7.5%. P...

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Thank you for contacting Abcam.
We have not specifically tested this antibody on immature and mature mucin. However, I have done some research. There's a paper which looks at O-glycosylation of MUC2. Specifically, this paper notices aberrant glyco...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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