Anti-MUC16抗体[X325] (ab10033)


  • 产品名称Anti-MUC16抗体[X325]
    参阅全部 MUC16 一抗
  • 描述
    小鼠单克隆抗体[X325] to MUC16
  • 特异性Epitope specificity group B (ISOBM classification).
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native protein (purified from human ovarian carcinoma).

  • 常规说明Concentration varies from lot to lot and can be provided on request.



Our Abpromise guarantee covers the use of ab10033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明ELISA: Use at an assay dependent dilution.
    IHC-P: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Detects a band of >1000 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
    • 组织特异性Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.
    • 序列相似性Contains 2 ANK repeats.
      Contains 56 SEA domains.
    • 结构域Composed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail.
    • 翻译后修饰Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
      Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
      May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
      May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.
    • 细胞定位Cell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CA 125 antibody
      • CA-125 antibody
      • CA125 antibody
      • CA125 ovarian cancer antigen antibody
      • Cancer antigen 125 antibody
      • FLJ14303 antibody
      • MUC 16 antibody
      • MUC-16 antibody
      • MUC16 antibody
      • MUC16_HUMAN antibody
      • Mucin 16 antibody
      • Mucin 16 cell surface associated antibody
      • Mucin-16 antibody
      • Mucin16 antibody
      • Ovarian cancer related tumor marker CA125 antibody
      • Ovarian cancer-related tumor marker CA125 antibody
      • Ovarian carcinoma antigen CA125 antibody
      see all

    Anti-MUC16 antibody [X325] 图像

    • ab10033 staining MUC16 in Human lung tissue (alveolocytes type II) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    • ab10033 staining MUC16 in Human benign ovarian tumor tissue (serous papillary cystadenoma) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    • ab10033 staining MUC16 in Human bladder carcinoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Anti-MUC16 antibody [X325] (ab10033)参考文献

    This product has been referenced in:
    • Shimizu A  et al. Coexpression of MUC16 and mesothelin is related to the invasion process in pancreatic ductal adenocarcinoma. Cancer Sci 103:739-46 (2012). Human . Read more (PubMed: 22320398) »
    • Kesimer M  et al. Molecular organization of the mucins and glycocalyx underlying mucus transport over mucosal surfaces of the airways. Mucosal Immunol : (2012). Read more (PubMed: 22929560) »

    See all 3 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Cynomolgus Monkey Tissue sections (Tonsil)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Ventana CC1
    Permeabilization No
    Specification Tonsil
    Blocking step Dako protein block as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
    Fixative Formaldehyde

    Sheng Zhong

    Verified customer

    提交于 Nov 24 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step Heat mediated
    Sample Human Tissue sections (urinary tract)
    Specification urinary tract
    Permeabilization No
    Fixative Formaldehyde

    Abcam user community

    Verified customer

    提交于 May 24 2013

    Thank you for contacting us.

    Following Western blot conditions have been tested with our anti-CA-125 MAbs:

    1. CA125 is immobilized on the nitrocellulose membrane for 30 min (400 mA, 25 mM Tris-Hcl, 192 mM Glycin, 0,1% SDS, 20% ethano...

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    I just wanted to let you know that I am still working on your inquiry. I have contacted the originator of this product for the information you requested, and I am awaiting a reply.

    I am v...

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    Thank you for your resposne.

    As my colleauge has explained in the previous e-mail, our antibodies and proteins will regrettably not have been tested and guaranteed in the application you are using (i.e. electrical biosensors).

    As EL...

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    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Human Cell lysate - whole cell (human colon and pancreas)
    Total protein in input 5000 µg
    Specification human colon and pancreas
    Immuno-precipitation step Protein G

    Abcam user community

    Verified customer

    提交于 Aug 02 2011