概述

  • 产品名称
    Anti-MUC16抗体[OC125]
    参阅全部 MUC16 一抗
  • 描述
    小鼠单克隆抗体[OC125] to MUC16
  • 特异性
    Studies have show that this antibody reacts with approximately 80% of epithelial ovarian neoplasms of serous, endometrioid, clear cell and undifferentiated types. No reactivity has been shown for mucinous ovarian tumors or in germ cell or hematopoietic tumors. It reacts with both normal tissues and neoplasms of fallopian tube, endometrium, endocervix and mesothelioma. It does not react with colon cancer. Normal tissues such as breast, liver, skin, kidney and spleen are negative.
  • 经测试应用
    适用于: Flow Cyt, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Partially purified human mucin fraction from a pool of tissues from patients with epithelial ovarian cancer.

  • 表位
    This antibody recognizes an epitope on a molecule called Cancer Antigen 125 (CA125).
  • 阳性对照
    • Flow cytometry: HeLa cells. IHC-P: Ovarian cancer tissue.

性能

应用

Our Abpromise guarantee covers the use of ab693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC Method.

靶标

  • 功能
    Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
  • 组织特异性
    Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.
  • 序列相似性
    Contains 2 ANK repeats.
    Contains 56 SEA domains.
  • 结构域
    Composed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail.
  • 翻译后修饰
    Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
    Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
    May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
    May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.
  • 细胞定位
    Cell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CA 125 antibody
    • CA-125 antibody
    • CA125 antibody
    • CA125 ovarian cancer antigen antibody
    • Cancer antigen 125 antibody
    • FLJ14303 antibody
    • MUC 16 antibody
    • MUC-16 antibody
    • MUC16 antibody
    • MUC16_HUMAN antibody
    • Mucin 16 antibody
    • Mucin 16 cell surface associated antibody
    • Mucin-16 antibody
    • Mucin16 antibody
    • Ovarian cancer related tumor marker CA125 antibody
    • Ovarian cancer-related tumor marker CA125 antibody
    • Ovarian carcinoma antigen CA125 antibody
    see all

Anti-MUC16 antibody [OC125] 图像

  • Overlay histogram showing HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained with ab693 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab693, 1 µg/1x106 cells) for 30 minutes at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized with 0.1% PBS-Tween for 20 minutes used under the same conditions.

  • Paraffin embedded human ovarian cancer tissue stained for MUC16 with ab693 (1/50 dilution) in immunohistochemical analysis.

Anti-MUC16 antibody [OC125] (ab693)参考文献

This product has been referenced in:
  • Kim BR  et al. SOX2 and PI3K Cooperate to Induce and Stabilize a Squamous-Committed Stem Cell Injury State during Lung Squamous Cell Carcinoma Pathogenesis. PLoS Biol 14:e1002581 (2016). ICC/IF ; Human . Read more (PubMed: 27880766) »
  • Zeng WJ  et al. Quantum dot-based multiplexed imaging in malignant ascites: a new model for malignant ascites classification. Int J Nanomedicine 10:1759-68 (2015). IHC-P ; Human . Read more (PubMed: 25784803) »

See all 10 Publications for this product

Product Wall

Thank you for your interest in the MUC16 antibody, clone OC125. The papers from 1981 and 1983 that are listed in the references section of the ab693 datasheet describe production and characterization of the antibody. The antigen was not a peptide; it w...

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The IgG concentration of the lot you have is 25.5 ug/mL. The antibody is not conjugated, so there is no F/P ratio.

Thank you for your reply. The concentration for the current lot is 50.5 ug/mL. Please let me know if you have any further questions.

Thank you for contacting us. The concentration is batch dependent. Have you already purchased the product? If so I can provide the concentration if you can send the lot number. Thank you!

I am very pleased to hear you would like to accept our offer and test ab693 in IP. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IP and include this code in the “Addition...

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Thank you for your reply and for clarifying the questions.

I can confirm that both these antibodies (ab693 and ab134093) are tested and covered by our 6 month guarantee for flow cytometry.

However, I am sorry they have not been tr...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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