重组
RabMAb

Anti-MUC16抗体[EPR1020(2)] (ab110640)

概述

  • 产品名称
    Anti-MUC16抗体[EPR1020(2)]
    参阅全部 MUC16 一抗
  • 描述
    兔单克隆抗体[EPR1020(2)] to MUC16
  • 经测试应用
    适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC/IF or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    corresponding to Human MUC16 aa 12250-12350.

  • 阳性对照
    • WB: Human uterus cell lysate. IHC-P: Human serous carcinoma and mucinous carcinoma tissues. ICC/IF: SK-OV-3 cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab110640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 1519 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 应用说明
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • 靶标

    • 功能
      Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
    • 组织特异性
      Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.
    • 序列相似性
      Contains 2 ANK repeats.
      Contains 56 SEA domains.
    • 结构域
      Composed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail.
    • 翻译后修饰
      Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
      Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
      May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
      May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.
    • 细胞定位
      Cell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CA 125 antibody
      • CA-125 antibody
      • CA125 antibody
      • CA125 ovarian cancer antigen antibody
      • Cancer antigen 125 antibody
      • FLJ14303 antibody
      • MUC 16 antibody
      • MUC-16 antibody
      • MUC16 antibody
      • MUC16_HUMAN antibody
      • Mucin 16 antibody
      • Mucin 16 cell surface associated antibody
      • Mucin-16 antibody
      • Mucin16 antibody
      • Ovarian cancer related tumor marker CA125 antibody
      • Ovarian cancer-related tumor marker CA125 antibody
      • Ovarian carcinoma antigen CA125 antibody
      see all

    Anti-MUC16 antibody [EPR1020(2)] 图像

    • Immunocytochemistry/Immunofluorescence analysis of SK-OV-3 cells labelling MUC16 with purified ab110640 at 1/500. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

      Control: PBS only.

    • ab110640 at 1/100 dilution staining MUC16 in Human serous carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

    • ab110640 at 1/100 dilution staining MUC16 in Human mucinous carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

    • ab110640 showing positive staining in Serous ovarian carcinoma tissue.

    • ab110640 showing positive staining in Normal uterus tissue.

    • ab110640 showing negative staining in Cervical carcinoma tissue.

    • ab110640 showing negative staining in Colonic adenocarcinoma tissue.

    • ab110640 showing negative staining in Normal kidney tissue.

    • ab110640 showing negative staining in Normal colon tissue.

    • ab110640 showing negative staining in Normal breast tissue.

    • ab110640 showing negative staining in Normal brain tissue.

    Anti-MUC16 antibody [EPR1020(2)] (ab110640)参考文献

    ab110640 has not yet been referenced specifically in any publications.

    Product Wall

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
    Sample
    Human Tissue sections (human endometrium)
    Specification
    human endometrium
    Permeabilization
    Yes - 0.2% Triton X-100 and PBS
    Fixative
    Formaldehyde
    Username

    Dr. Steffen Rickelt

    Verified customer

    提交于 Mar 17 2014

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (human ovarian cystedenocarcinoma tissue sections)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
    Permeabilization
    No
    Specification
    human ovarian cystedenocarcinoma tissue sections
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Aug 03 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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