Anti-MUC13抗体(ab65109)
Key features and details
- Rabbit polyclonal to MUC13
- Suitable for: ELISA, IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-MUC13抗体
参阅全部 MUC13 一抗 -
描述
兔多克隆抗体to MUC13 -
宿主
Rabbit -
经测试应用
适用于: ELISA, IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide (Human) from an internal sequence.
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阳性对照
- WB: Extracts from COLO, 293 and HeLa cells. IHC-P: Human colon carcinoma tissue. IF: HepG2 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab65109于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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IHC-P | (1) |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
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ICC/IF |
1/500 - 1/1000.
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说明 |
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ELISA
Use at an assay dependent concentration. |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
ICC/IF
1/500 - 1/1000. |
靶标
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功能
Epithelial and hemopoietic transmembrane mucin that may play a role in cell signaling. -
组织特异性
Highly expressed in epithelial tissues, particularly those of the gastrointestinal and respiratory tracts, such as large intestine and trachea, followed by kidney, small intestine, appendix and stomach. -
序列相似性
Contains 3 EGF-like domains.
Contains 1 SEA domain. -
翻译后修饰
Cleaved into two subunits, alpha and beta, probably between the first EGF domain and the SEA domain. Beta subunit contains the cytoplasmic tail and alpha subunit the extracellular tail. The homo-oligomerization into dimers is dependent on intrachain disulfide bonds.
Highly N-glycosylated. -
细胞定位
Cell membrane. Secreted. Also exists as a soluble form. - Information by UniProt
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数据库链接
- Entrez Gene: 56667 Human
- Omim: 612181 Human
- SwissProt: Q9H3R2 Human
- Unigene: 5940 Human
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别名
- Down-regulated in colon cancer 1 antibody
- DRCC1 antibody
- FLJ20063 antibody
see all
图片
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Immunofluorescence analysis of HepG2 cells, using MUC13 antibody. The picture on the right is blocked with synthesized peptide.
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All lanes : Anti-MUC13 antibody (ab65109) at 1/500 dilution
Lane 1 : extracts from COLO cells
Lane 2 : extracts from 293 cells
Lane 3 : extracts from HeLa cells
Lane 4 : extracts from HeLa cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 55 kDa
Observed band size: 55 kDaSecondary antibody - anti-rabbit HRP (ab6721)
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Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labelling MUC13 with ab65109.
The picture on the right is blocked with the synthesized peptide. -
ICC/IF image of ab65109 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65109, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab65109 被引用在 5 文献中.
- Pothuraju R et al. Depletion of transmembrane mucin 4 (Muc4) alters intestinal homeostasis in a genetically engineered mouse model of colorectal cancer. Aging (Albany NY) 14:2025-2046 (2022). PubMed: 35255004
- Lv J et al. Gasdermin E mediates resistance of pancreatic adenocarcinoma to enzymatic digestion through a YBX1-mucin pathway. Nat Cell Biol 24:364-372 (2022). PubMed: 35292781
- LaMonte GM et al. Dual RNA-seq identifies human mucosal immunity protein Mucin-13 as a hallmark of Plasmodium exoerythrocytic infection. Nat Commun 10:488 (2019). PubMed: 30700707
- Journo G et al. Modulation of Cellular CpG DNA Methylation by Kaposi's Sarcoma-Associated Herpesvirus. J Virol 92:N/A (2018). PubMed: 29899086
- Jepsen CS et al. FIBCD1 Binds Aspergillus fumigatus and Regulates Lung Epithelial Response to Cell Wall Components. Front Immunol 9:1967 (2018). PubMed: 30279687