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Our Abpromise guarantee covers the use of ab124654 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1999. Predicted molecular weight: 55 kDa.|
|IHC-P||Use a concentration of 5 µg/ml.|
|ICC/IF||1/100 - 1/500.|
ab124654 staining MUC13 in mouse colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked for 30 minutes at room temperature. Samples were incubated with primary antibody (1/25 in PBS Tween) for 1 hour. An undiluted HRP polymer-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"