重组Anti-Mu Opioid Receptor抗体[UMB3] - Low endotoxin,Azide free (ab227067)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [UMB3] to Mu Opioid Receptor - Low endotoxin, Azide free
- Suitable for: WB
- Reacts with: Human
概述
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产品名称
Anti-Mu Opioid Receptor抗体[UMB3] - Low endotoxin,Azide free
参阅全部 Mu Opioid Receptor 一抗 -
描述
兔单克隆抗体[UMB3] to Mu Opioid Receptor - Low endotoxin,Azide free -
宿主
Rabbit -
特异性
The antibody detects the band of interest in transfected cell lines. When tested in our lab, we were not able to detect any signal from the endogenous protein.This antibody can be used in IHC and ICC/IF as demonstrated in several publications, however, it did not pass our internal validation in these applications. Please refer to PMID 20851148, 21957251, 26290245, and other references.
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经测试应用
适用于: WBmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Mu Opioid Receptor transfected HEK293 lysate.
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常规说明
ab227067 is the carrier-free version of ab134054.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
UMB3 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab227067于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 75-78 kDa (predicted molecular weight: 45 kDa).
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说明 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 75-78 kDa (predicted molecular weight: 45 kDa). |
靶标
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功能
Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for beta-endorphin. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4988 Human
- Entrez Gene: 18390 Mouse
- Entrez Gene: 25601 Rat
- Omim: 600018 Human
- SwissProt: P35372 Human
- SwissProt: P42866 Mouse
- SwissProt: P33535 Rat
- Unigene: 2353 Human
see all -
别名
- hMOP antibody
- LMOR antibody
- M-OR-1 antibody
see all
图片
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All lanes : Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054) at 1/1000 dilution
Lane 1 : Mu Opioid Receptor transfected HEK-293 (Human epithelial cell line from embryonic kidney) lysate
Lane 2 : HEK-293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 45 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (9)
ab227067 被引用在 9 文献中.
- Vatter T et al. Against Repurposing Methadone for Glioblastoma Therapy. Biomolecules 10:N/A (2020). PubMed: 32560384
- Crittenden JR et al. Striosome-dendron bouquets highlight a unique striatonigral circuit targeting dopamine-containing neurons. Proc Natl Acad Sci U S A 113:11318-11323 (2016). IHC-Fr ; Mouse . PubMed: 27647894
- Ozawa A et al. Knock-In Mice with NOP-eGFP Receptors Identify Receptor Cellular and Regional Localization. J Neurosci 35:11682-93 (2015). PubMed: 26290245
- Hassan B et al. Alteration of the mu opioid receptor: Ca2+ channel signaling pathway in a subset of rat sensory neurons following chronic femoral artery occlusion. J Neurophysiol 112:3104-15 (2014). PubMed: 25231620
- Just S et al. Differentiation of opioid drug effects by hierarchical multi-site phosphorylation. Mol Pharmacol 83:633-9 (2013). PubMed: 23239825
- Gallagher SK et al. Dopaminergic amacrine cells express opioid receptors in the mouse retina. Vis Neurosci 29:203-9 (2012). WB ; Mouse . PubMed: 22643230
- Lupp A et al. UMB-3, a novel rabbit monoclonal antibody, for assessing µ-opioid receptor expression in mouse, rat and human formalin-fixed and paraffin-embedded tissues. Regul Pept 167:9-13 (2011). PubMed: 20851148
- Doll C et al. Agonist-selective patterns of µ-opioid receptor phosphorylation revealed by phosphosite-specific antibodies. Br J Pharmacol 164:298-307 (2011). PubMed: 21449911
- Grecksch G et al. Analgesic tolerance to high-efficacy agonists but not to morphine is diminished in phosphorylation-deficient S375A µ-opioid receptor knock-in mice. J Neurosci 31:13890-6 (2011). IP, IHC-P ; Mouse . PubMed: 21957251