The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 44 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Multiple alternatively spliced transcript variants encoding different isoforms have been found for the MTUS1 (Mitochondrial tumor suppressor 1) gene. One of the transcript variants has been shown to encode a mitochondrial protein that acts as a tumor suppressor and cooperates with the angiotension II (AT2) receptor (AGTR2) to inhibit ERK2 activation and cell proliferation. MTUS1 polypeptides are identical in their carboxy-terminal region carrying four coiled-coil domains. In their amino-terminal portion, MTUS1 polypeptides exhibit distinct motifs for localisation in the cytosol, nucleus or cell membrane, suggesting that MTUS1 gene products may be involved in a variety of intracellular functions in an AT2-dependent and independent manner.
Cell Membrane, Golgi Apparatus, Mitochondrial and Nuclear. Note=In neurons, translocates into the nucleus after treatment with angiotensin-II.
All lanes : Anti-MTUS1 antibody (ab61309) at 1/200 dilution
Lane 1 : Whole cell lysate prepared from MCF cells transfected with GFP-ATIP1 Lane 2 : Whole cell lysate prepared from MCF cells transfected with GFP-ATIP3 Lane 3 : Whole cell lysate prepared from MCF cells transfected with GFP