概述

  • 产品名称Anti-MTTP抗体
    参阅全部 MTTP 一抗
  • 描述
    兔多克隆抗体to MTTP
  • 特异性Reactivity with human samples appears to be lot-dependent. A user reported strong western blot reactivity with a mouse cell lysate but none with a human HepG2 lysate.
  • 经测试应用适用于: WB, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Synthetic peptide derived from C-terimal domain of mouse MTTP

性能

应用

Our Abpromise guarantee covers the use of ab75316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/200 - 1/2000. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能Catalyzes the transport of triglyceride, cholesteryl ester, and phospholipid between phospholipid surfaces. Required for the secretion of plasma lipoproteins that contain apolipoprotein B.
  • 组织特异性Liver and small intestine. Also found in ovary, testis and kidney.
  • 疾病相关Abetalipoproteinemia
  • 序列相似性Contains 1 vitellogenin domain.
  • 细胞定位Endoplasmic reticulum.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ABL antibody
    • Microsomal triglyceride transfer protein (large polypeptide 88kDa) antibody
    • Microsomal triglyceride transfer protein 88kD antibody
    • Microsomal triglyceride transfer protein antibody
    • Microsomal triglyceride transfer protein large subunit antibody
    • MTP antibody
    • MTP large subunit antibody
    • MTP_HUMAN antibody
    • MTTP antibody
    • Transfer RNA, mitochondrial, proline antibody
    • tRNA-Pro, mitochondrial antibody
    see all

Anti-MTTP antibody 图像

  • All lanes : Anti-MTTP antibody (ab75316) at 1/500 dilution

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Liver (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 99 kDa
    Observed band size : 99 kDa
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

Anti-MTTP antibody (ab75316)参考文献

This product has been referenced in:
  • Hesse D  et al. Hepatic trans-Golgi action coordinated by the GTPase ARFRP1 is crucial for lipoprotein lipidation and assembly. J Lipid Res 55:41-52 (2014). WB ; Mouse . Read more (PubMed: 24186947) »
  • Lee LY  et al. Activation of the Nrf2-ARE pathway in hepatocytes protects against steatosis in nutritionally induced non-alcoholic steatohepatitis in mice. Toxicol Sci 142:361-74 (2014). Read more (PubMed: 25294219) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Hamster Tissue lysate - whole (Hamster (Mesocricetus auratus) small intestine)
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Loading amount 75 µg
Treatment Western diet (N normal, HL hyperlipemic), 16 weeks
Specification Hamster (Mesocricetus auratus) small intestine
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Anca Sima

Verified customer

提交于 May 21 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"