概述

  • 产品名称Anti-mtRNA polymerase抗体
    参阅全部 mtRNA polymerase 一抗
  • 描述
    兔多克隆抗体to mtRNA polymerase
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性

    预测可用于: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1200 to the C-terminus of Human mtRNA polymerase.

    (Peptide available as ab32987.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates; HeLa, HEK293 and Caco2.

性能

应用

Our Abpromise guarantee covers the use of ab32988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB 1/250. Detects a band of approximately 140 kDa (predicted molecular weight: 139 kDa).

靶标

  • 功能DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates.
  • 序列相似性Belongs to the phage and mitochondrial RNA polymerase family.
  • 细胞定位Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APOLMT antibody
    • DNA-directed RNA polymerase antibody
    • DNA-directed RNA polymerase mitochondrial antibody
    • h-mtRPOL antibody
    • mitochondrial antibody
    • MTRNAP antibody
    • MtRPOL antibody
    • POLRMT antibody
    • polymerase (RNA) mitochondrial (DNA directed) antibody
    • polymerase, RNA, mitochondrial antibody
    • RPOM antibody
    • RPOM_HUMAN antibody
    see all

Anti-mtRNA polymerase antibody 图像

  • All lanes : Anti-mtRNA polymerase antibody (ab32988) at 1/250 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 139 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 160 kDa,45 kDa,65 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab32988 stained HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab32988, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in 4% PFA fixed MCF7 cells.

Anti-mtRNA polymerase antibody (ab32988)参考文献

This product has been referenced in:
  • Nouws J  et al. Mitochondrial Ribosomal Protein L12 Is Required for POLRMT Stability and Exists as Two Forms Generated by Alternative Proteolysis during Import. J Biol Chem 291:989-97 (2016). Read more (PubMed: 26586915) »
  • Harmel J  et al. The leucine-rich pentatricopeptide repeat-containing protein (LRPPRC) does not activate transcription in mammalian mitochondria. J Biol Chem 288:15510-9 (2013). WB ; Mouse . Read more (PubMed: 23599432) »

See all 4 Publications for this product

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As discussed please remove the precipitates by centrifuging the vial and use it in western blot. If the antibody fails please do not hesitate to contact me for replacement.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"