概述

  • 产品名称
    Anti-mtRNA polymerase抗体
    参阅全部 mtRNA polymerase 一抗
  • 描述
    兔多克隆抗体to mtRNA polymerase
  • 经测试应用
    适用于: WBmore details
  • 种属反应性

    预测可用于: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1200 to the C-terminus of Human mtRNA polymerase.

    (Peptide available as ab32987.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates; HeLa, HEK293 and Caco2.

性能

应用

Our Abpromise guarantee covers the use of ab32988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250. Detects a band of approximately 140 kDa (predicted molecular weight: 139 kDa).

靶标

  • 功能
    DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates.
  • 序列相似性
    Belongs to the phage and mitochondrial RNA polymerase family.
  • 细胞定位
    Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APOLMT antibody
    • DNA-directed RNA polymerase antibody
    • DNA-directed RNA polymerase mitochondrial antibody
    • h-mtRPOL antibody
    • mitochondrial antibody
    • MTRNAP antibody
    • MtRPOL antibody
    • POLRMT antibody
    • polymerase (RNA) mitochondrial (DNA directed) antibody
    • polymerase, RNA, mitochondrial antibody
    • RPOM antibody
    • RPOM_HUMAN antibody
    see all

图片

  • All lanes : Anti-mtRNA polymerase antibody (ab32988) at 1/250 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 139 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 160 kDa,45 kDa,65 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

This product has been referenced in:
  • Nouws J  et al. Mitochondrial Ribosomal Protein L12 Is Required for POLRMT Stability and Exists as Two Forms Generated by Alternative Proteolysis during Import. J Biol Chem 291:989-97 (2016). Read more (PubMed: 26586915) »
  • Bralha FN  et al. Targeting mitochondrial RNA polymerase in acute myeloid leukemia. Oncotarget 6:37216-28 (2015). WB . Read more (PubMed: 26484416) »

See all 5 Publications for this product

客户评价及客户问答

Vielen Dank für Ihren Anruf vom letzten Freitag.

Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten.

Wir haben nunalle Röhrchen die wir an Lager haben überprüft. In keinem unserer Röhrchen...

Read More

Thank you for contacting us.

As discussed please remove the precipitates by centrifuging the vial and use it in western blot. If the antibody fails please do not hesitate to contact me for replacement.

Have a nice day!

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册