概述

  • 产品名称Anti-MTR抗体
    参阅全部 MTR 一抗
  • 描述
    兔多克隆抗体to MTR
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Caenorhabditis elegans
    预测可用于: Horse, Pig, Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1200 to the C-terminus of Human MTR.

    (Peptide available as ab69085.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: TE 671 Whole Cell, Mouse Liver Tissue, Rat Thymus Tissue, Mouse Pancreas Tissue; Jurkat Whole Cell - Staurosporine Treated (24hr, 500nM), HeLa Whole Cell - Staurosporine Treated (24hr, 500nM), HeLa Whole Cell - Bleomycin Treated (20U/ml), HeLa Whole Cell, Mouse Kidney Tissue, Rat Liver Tissue

性能

应用

Our Abpromise guarantee covers the use of ab66039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 141 kDa (predicted molecular weight: 141 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 相关性MTR encodes the enzyme 5-methyltetrahydrofolate-homocysteine methyltransferase. This enzyme, also known as cobalamin-dependent methionine synthase, catalyzes the final step in methionine biosynthesis. Mutations in MTR have been identified as the underlying cause of methylcobalamin deficiency complementation group G.
  • 细胞定位Cytoplasmic
  • 数据库链接
  • 别名
    • 5-methyltetrahydrofolate homocysteine methyltransferase antibody
    • 5-methyltetrahydrofolate-homocysteine methyltransferase antibody
    • cblG antibody
    • Methionine synthase antibody
    • methioninesynthase antibody
    • MS antibody
    • MTR antibody
    • MTR1 antibody
    • PRP20 antibody
    • SRM1 antibody
    • Vitamin-B12 dependent methionine synthase antibody
    see all

Anti-MTR antibody 图像

  • All lanes : Anti-MTR antibody (ab66039) at 1 µg/ml

    Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : Thymus (Rat) Tissue Lysate
    Lane 4 : Pancreas (Mouse) Tissue Lysate
    Lane 5 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
    Lane 6 : HeLa Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
    Lane 7 : HeLa Whole Cell Lysate - Bleomycin Treated (20U/ml)
    Lane 8 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 9 : Kidney (Mouse) Tissue Lysate
    Lane 10 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 141 kDa
    Observed band size : 141 kDa
    Additional bands at : 117 kDa,35 kDa,50 kDa,55 kDa,65 kDa,85 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab66039 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66039, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, Hek293 and HepG2 cells at 5µg/ml, and in 4% PFA fixed (10 min) MCF7 cells at 5µg/ml.
  • IHC image of ab66039 staining in pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab66039, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-MTR antibody (ab66039)参考文献

This product has been referenced in:
  • Bito T  et al. A dodecylamine derivative of cyanocobalamin potently inhibits the activities of cobalamin-dependent methylmalonyl-CoA mutase and methionine synthase of Caenorhabditis elegans. FEBS Open Bio 4:722-9 (2014). WB ; Caenorhabditis elegans . Read more (PubMed: 25161880) »
  • Pérez-Sepúlveda A  et al. Levels of key enzymes of methionine-homocysteine metabolism in preeclampsia. Biomed Res Int 2013:731962 (2013). WB ; Human . Read more (PubMed: 24024209) »

See all 4 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Non-reduced Denaturing (10%)
Sample Mouse Tissue lysate - whole (Liver)
Specification Liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Apr 10 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"