Anti-mTOR (phospho S2448)抗体(ab1093)

概述

  • 产品名称Anti-mTOR (phospho S2448)抗体
    参阅全部 mTOR 一抗
  • 描述
    兔多克隆抗体to mTOR (phospho S2448)
  • 经测试应用适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide: KRSRTRTDpSYSAGQSVEI, corresponding to C terminal amino acids 2440-2463 of Human mTOR.

  • 阳性对照
    • serum starved 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab1093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB 1/1000. Detects a band of approximately 250 kDa (predicted molecular weight: 288 kDa).
ELISA 1/6000 - 1/29000. Assayed aginst 0.1 µg of the immunizing peptide and has not been tested against the endogenous protein.

靶标

  • 功能Kinase subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Phosphorylates MAF1 leading to attenuation of its RNA polymerase III-repressive function. mTORC2 is also activated by growth. factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
  • 组织特异性Expressed in numerous tissues, with highest levels in testis.
  • 序列相似性Belongs to the PI3/PI4-kinase family.
    Contains 1 FAT domain.
    Contains 1 FATC domain.
    Contains 7 HEAT repeats.
    Contains 1 PI3K/PI4K domain.
  • 翻译后修饰Autophosphorylated; when part of mTORC1 or mTORC2.
  • 细胞定位Endoplasmic reticulum membrane. Golgi apparatus membrane. Mitochondrion outer membrane. Lysosome. Cytoplasm. Nucleus > PML body. Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB.
  • Information by UniProt
  • 数据库链接
  • 别名
    • dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) antibody
    • FK506 binding protein 12 rapamycin associated protein 1 antibody
    • FK506 binding protein 12 rapamycin associated protein 2 antibody
    • FK506 binding protein 12 rapamycin complex associated protein 1 antibody
    • FK506-binding protein 12-rapamycin complex-associated protein 1 antibody
    • FKBP rapamycin associated protein antibody
    • FKBP12 rapamycin complex associated protein antibody
    • FKBP12-rapamycin complex-associated protein 1 antibody
    • FKBP12-rapamycin complex-associated protein antibody
    • FLJ44809 antibody
    • FRAP antibody
    • FRAP1 antibody
    • FRAP2 antibody
    • Mammalian target of rapamycin antibody
    • Mechanistic target of rapamycin antibody
    • mTOR antibody
    • MTOR_HUMAN antibody
    • OTTHUMP00000001983 antibody
    • RAFT1 antibody
    • Rapamycin and FKBP12 target 1 antibody
    • Rapamycin associated protein FRAP2 antibody
    • Rapamycin target protein 1 antibody
    • Rapamycin target protein antibody
    • RAPT1 antibody
    • Serine/threonine-protein kinase mTOR antibody
    see all

Anti-mTOR (phospho S2448) antibody 图像



  • Predicted band size : 288 kDa

    This image is courtesy of Angela Carter, Experimental Therapeutics, Ontario Cancer Inst, Toronto, Canada

    Rabbit polyclonal to phospho mTOR (Ser 2448) at 1/1000 on 293T cell extract shows a band at approx 250 kDa.
    Cells were serum starved for 24 hours:

    Lane 1 : control
    Lane 2 : treated with IGF-1 (100 ng/ml) for 20 min

    Rabbit polyclonal to phospho mTOR (Ser 2448) at 1/1000 on 293T cell extract shows a band at approx 250 kDa. Cells were serum starved for 24 hours:
    Lane 1 : control
    Lane 2 : treated with IGF-1 (100 ng/ml) for 20 min

  • ab1093 at 5 µg/ml staining proximal convulted tubules of the kidney.  The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

Anti-mTOR (phospho S2448) antibody (ab1093)参考文献

This product has been referenced in:
  • Wyszynski RW  et al. Interleukin-1 beta induces the expression and production of stem cell factor by epithelial cells: crucial involvement of the PI-3K/mTOR pathway and HIF-1 transcription complex. Cell Mol Immunol N/A:N/A (2014). Read more (PubMed: 25418470) »
  • Xu L  et al. MicroRNA-7-regulated TLR9 signaling-enhanced growth and metastatic potential of human lung cancer cells by altering the phosphoinositide-3-kinase, regulatory subunit 3/Akt pathway. Mol Biol Cell 24:42-55 (2013). WB . Read more (PubMed: 23135998) »

See all 3 Publications for this product

Product Wall

I'm sorry to hear one of your customers is experiencing a problem with ab1093. We have not tested this antibody in cultured HepG2 , HCT116, DLD1, SUV480 and HT29 cells and I think the customer's problem is due to the fact that these cells contain lo...

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