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Our Abpromise guarantee covers the use of ab55530 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Can be blocked with Recombinant MTHFR protein (ab152003).
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
|ELISA||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 23525608|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 22419888|