概述

  • 产品名称Anti-MTHFD2抗体
    参阅全部 MTHFD2 一抗
  • 描述
    小鼠单克隆抗体to MTHFD2
  • 经测试应用适用于: WB, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 16-345 of Human MTHFD2

性能

应用

Our Abpromise guarantee covers the use of ab56772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 37 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 序列相似性Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.
  • 发展阶段Expressed only in developing normal tissues.
  • 细胞定位Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial antibody
    • Descriptions antibody
    • Methenyltetrahydrofolate cyclohydrolase antibody
    • methylene tetrahydrofolate dehydrogenase (NAD+ dependent) antibody
    • methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2 antibody
    • methylenetetrahydrofolate dehydrogenase (NADP+ dependent) antibody
    • methylenetetrahydrofolate dehydrogenase 2 antibody
    • MGC82516 antibody
    • MTDC_HUMAN antibody
    • MTHFD2 antibody
    • mthfd2 methylene tetrahydrofolate dehydrogenase (NAD+ dependent) antibody
    • NAD-dependent methylene tetrahydrofolate dehydrogenase antibody
    • NAD-dependent methylenetetrahydrofolate dehydrogenase antibody
    • NMDMC antibody
    see all

Anti-MTHFD2 antibody 图像

  • MTHFD2 antibody (ab56772) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lymphoma tissue.


  • Predicted band size : 37 kDa
    MTHFD2 antibody (ab56772) at 1ug/lane + HepG2 cell lysate at 25ug/lane.
  • Overlay histogram showing HepG2 cells stained with ab56772 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56772, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-MTHFD2 antibody (ab56772)参考文献

This product has been referenced in:
  • Nilsson R  et al. Metabolic enzyme expression highlights a key role for MTHFD2 and the mitochondrial folate pathway in cancer. Nat Commun 5:3128 (2014). WB ; Human . Read more (PubMed: 24451681) »

See 1 Publication for this product

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