概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to MTHFD1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human MTHFD1 protein (AAH50420.1).

  • 阳性对照
    • MTHFD1 transfected 293T cell lysate, human placenta cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab70203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 102 kDa).

靶标

  • 组织特异性
    Ubiquitous.
  • 通路
    One-carbon metabolism; tetrahydrofolate interconversion.
  • 疾病相关
    Defects in MTHFD1 may be a cause of susceptibility to folate-sensitive neural tube defects (folate-sensitive NTD) [MIM:601634]. The most common NTDs are open spina bifida (myelomeningocele) and anencephaly. Genetic defects in MTHFD1 may affect the risk of spina bifida via the maternal rather than the embryonic genotype.
    Genetic variation in MTHFD1 could be associated with susceptibility to colorectal cancer (CRC) [MIM:114500].
  • 序列相似性
    In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.
    In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.
  • 结构域
    This trifunctional enzyme consists of two major domains: an N-terminal part containing the methylene-THF dehydrogenase and cyclohydrolase activities and a larger C-terminal part containing formyl-THF synthetase activity.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 5,10 methylenetetrahydrofolate dehydrogenase 5,10 methylenetetrahydrofolate cyclohydrolase,10 formyltetrahydrofolate synthetase antibody
    • C 1 tetrahydrofolate synthase cytoplasmic antibody
    • C1 tetrahydrofolate synthase cytoplasmic antibody
    • C1 THF synthase antibody
    • C1-THF synthase antibody
    • C1TC antibody
    • C1TC_HUMAN antibody
    • Cytoplasmic C 1 tetrahydrofolate synthase antibody
    • Formyltetrahydrofolate synthetase antibody
    • Methenyltetrahydrofolate cyclohydrolase antibody
    • Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) methenyltetrahydrofolate cyclohydrolase formyltetrahydrofolate synthetase antibody
    • Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 methenyltetrahydrofolate cyclohydrolase formyltetrahydrofolate synthetase antibody
    • Methylenetetrahydrofolate dehydrogenase antibody
    • MTHFC antibody
    • MTHFD antibody
    • MTHFD1 antibody
    • NADP dependent cyclohydrolase formyltetrahydrofolate synthetase antibody
    • OTTHUMP00000180657 antibody
    see all

图片

  • Anti-MTHFD1 antibody (ab70203) at 1/500 dilution + human placenta at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 102 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-MTHFD1 antibody (ab70203) at 1/500 dilution

    Lane 1 : MTHFD1 transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 102 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab70203 has not yet been referenced specifically in any publications.

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