概述

  • 产品名称
  • 描述
    小鼠单克隆抗体to MTF1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: GTVYDRTTVL IEQDPGTLED EDDDGQCGEH LPFLVGGEEG FHLIDHEAMS QGYVQHIISP DQIHLTINPG STPMPRNIEG ATLTLQSECP ETKRKEVKRY , corresponding to amino acids 41-141 of Human MTF1

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 同种型
    IgG2a
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab55522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml.

Please see specific references PMID: 22021037 and 18990686 regarding use of this antibody to detect endogenous human MTF1.

靶标

  • 功能
    Activates the metallothionein I promoter. Binds to the metal responsive element (MRE).
  • 序列相似性
    Contains 6 C2H2-type zinc fingers.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Metal regulatory transcription factor 1 antibody
    • Metal response element binding transcription factor 1 antibody
    • Metal responsive transcription factor 1 antibody
    • MRE binding transcription factor antibody
    • MRE-binding transcription factor 1 antibody
    • MRE-binding transcription factor antibody
    • MTF 1 antibody
    • MTF1 antibody
    • MTF1_HUMAN antibody
    • Transcription factor MTF-1 antibody
    • Transcription factor MTF1 antibody
    • Zinc regulatory factor antibody
    • ZRF antibody
    see all

图片

  • Western blot against tagged recombinant protein immunogen using ab55522 MTF1 antibody at 1ug/ml. Predicted band size of the recombinant immunogen is 37 kDa.
    We have only tested this antibody against the reecombinant protein.
    Please see specific references PMID: 22021037 and 18990686 regarding use of this antibody to detect endogenous human MTF1.

实验方案

文献

This product has been referenced in:
  • Liu YC  et al. The Role of Small Ubiquitin-like Modifier-interacting Motif in the Assembly and Regulation of Metal-responsive Transcription Factor 1. J Biol Chem 286:42818-29 (2011). WB ; Human . Read more (PubMed: 22021037) »
  • Bellingham SA  et al. Regulation of prion gene expression by transcription factors SP1 and metal transcription factor-1. J Biol Chem 284:1291-301 (2009). WB ; Human . Read more (PubMed: 18990686) »

See all 2 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (human MDM)
Loading amount
100 µg
Specification
human MDM
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

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Verified customer

提交于 Jan 28 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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