概述

  • 产品名称
    Anti-MTERFD3抗体
    参阅全部 MTERFD3 一抗
  • 描述
    小鼠多克隆抗体to MTERFD3
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Recombinant fragment:

    AAVNTKRKLWQMVCKNEAEL

    , corresponding to amino acids 110-129 of Mouse MTERFD3

  • 常规说明


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 50% Glycerol, Whole serum
  • 纯度
    Whole antiserum
  • Primary antibody说明
    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab52692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 44 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 功能
    Binds promoter DNA and regulates mitochondrial transcription. Required for normal levels of transcription, both for mRNA and tRNA. Required for normal mitchondrial protein synthesis, assembly of respiratory complexes and normal mitochondrial function.
  • 组织特异性
    Expressed in skeletal muscle, heart, liver and pancreas.
  • 序列相似性
    Belongs to the mTERF family.
  • 细胞定位
    Mitochondrion. Mitochondrion matrix > mitochondrion nucleoid.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Hypothetical protein DKFZp667I1824 antibody
    • mitochondrial antibody
    • Mitochondrial transcription termination factor 2 antibody
    • Mitochondrial transcription termination factor-like protein antibody
    • MTER3_HUMAN antibody
    • MTERF domain containing 3 antibody
    • mTERF domain-containing protein 3 antibody
    • mTERF domain-containing protein 3, mitochondrial antibody
    • mTERF2 antibody
    • Mterfd3 antibody
    • mTERFL antibody
    • Transcription termination factor 2 mitochondrial antibody
    • Transcription termination factor like protein antibody
    see all

Anti-MTERFD3 antibody 图像

  • All lanes : Anti-MTERFD3 antibody (ab52692) at 1/1000 dilution

    Lane 1 : Left: The negative control lane of ~20ug of a total protein extract with ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen
    Lane 2 : Right: test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein)

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 44 kDa
    Observed band size : 32.2 kDa (why is the actual band size different from the predicted?)

实验方案

Anti-MTERFD3 antibody (ab52692)参考文献

ab52692 has not yet been referenced specifically in any publications.

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