antigen sequence, corresponding to N terminal amino acids 23-152 of Human MTERFD2.
- MTERFD2 over-expression HEK293T cell lysate; Human skeletal muscle tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/250 - 1/500.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Anti-MTERFD2 antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-MTERFD2 antibody (ab121910)
Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoplasm and mitochondria. Recommended concentration of ab121910 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-MTERFD2 antibody (ab121910)
All lanes : Anti-MTERFD2 antibody (ab121910) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : MTERFD2 over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTERFD2 antibody (ab121910)
ab121910, at 1/60 dilution, staining MTERFD2 in paraffin embedded Human skeletal muscle tissue by Immunohistochemistry.
Anti-MTERFD2 antibody (ab121910)参考文献
has not yet been referenced specifically in any publications.
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