Anti-MTCO3抗体[DA5BC4] (ab110259)

概述

  • 产品名称
    Anti-MTCO3抗体[DA5BC4]
    参阅全部 MTCO3 一抗
  • 描述
    小鼠单克隆抗体[DA5BC4] to MTCO3
  • 经测试应用
    适用于: IP, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human, Saccharomyces cerevisiae
  • 免疫原

    Full length native Cytochrome C Oxidase subunit III (Saccharomyces cerevisiae).

  • 阳性对照
    • Isolated mitochondria from Human , Rat and Mouse hearts.
  • 常规说明

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

应用

Our Abpromise guarantee covers the use of ab110259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at 10-1 µg/mg of lysate.
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 30 kDa.

靶标

  • 功能
    Subunits I, II and III form the functional core of the enzyme complex.
  • 疾病相关
    Defects in MT-CO3 are a cause of Leber hereditary optic neuropathy (LHON) [MIM:535000]. LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes.
    Defects in MT-CO3 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
    Defects in MT-CO3 are associated with recurrent myoglobinuria mitochondrial (RM-MT) [MIM:550500]. Recurrent myoglobinuria is characterized by recurrent attacks of rhabdomyolysis (necrosis or disintegration of skeletal muscle) associated with muscle pain and weakness, and followed by excretion of myoglobin in the urine.
  • 序列相似性
    Belongs to the cytochrome c oxidase subunit 3 family.
  • 细胞定位
    Mitochondrion inner membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • COIII antibody
    • COX3 antibody
    • COX3_HUMAN antibody
    • COXIII antibody
    • Cytochrome c oxidase polypeptide III antibody
    • Cytochrome c oxidase subunit 3 antibody
    • MT CO3 antibody
    • MT-CO3 antibody
    • MTCO3 antibody
    see all

图片

  • All lanes : Anti-MTCO3 antibody [DA5BC4] (ab110259) at 4 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria Rat heart at 10 µg
    Lane 3 : Isolated mitochondria from Mouse heart at 10 µg


    Predicted band size : 30 kDa

文献

This product has been referenced in:
  • Zhao C  et al. Geniposide Protects Primary Cortical Neurons against Oligomeric Aß1-42-Induced Neurotoxicity through a Mitochondrial Pathway. PLoS One 11:e0152551 (2016). WB ; Mouse . Read more (PubMed: 27046221) »
  • Swenson S  et al. Analysis of Oligomerization Properties of Heme a Synthase Provides Insights into Its Function in Eukaryotes. J Biol Chem 291:10411-25 (2016). Read more (PubMed: 26940873) »

See all 23 Publications for this product

客户评价及客户问答

Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Unserem Wissen nach wurde der Anti-Cytochrome C Oxidase subunit III antibody [DA5BC4] (ab110259) bisher noch nicht in IHC-Fr getestet.

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Die gute Nachricht ist, dass wir noch drei Röhrchen der Charge GR87744 des ab110259 Anti-Cytochrome C Oxidase subunit III antibody [DA5BC4]habe. Die schlechte ist, dass sich zwei davon in unser...

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Thank you for your email.

We have reserved four vials of lot x for you. It has currently the order number x- please refer to this number when placing the order. Thank you.

I have also meanwhile heard back from the laboratory. The la...

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Application
Western blot
Sample
Human Cell lysate - other (HeLa WT vs HeLa rho-0)
Loading amount
13 µg
Specification
HeLa WT vs HeLa rho-0
Gel Running Conditions
Reduced Denaturing (Reduced Denaturing (4-15%))
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jul 27 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - other (Mouse Liver Mitochondria)
Loading amount
20 µg
Specification
Mouse Liver Mitochondria
Gel Running Conditions
Reduced Denaturing (10% Bis Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 01 2011

Thank you for contacting Abcam. I will be glad to help you with your inquiry for a positive control for your antibodies. We do have several mitochondrial control lysates, which I have listed below: Mouse mitochondrial liver lysate...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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